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Titolo:
OVEREXPRESSION OF P53 PROTEIN IS COMMON IN PREMALIGNANT HEAD AND NECKLESIONS
Autore:
PAVELIC ZP; LI YQ; STAMBROOK PJ; MCDONALD JS; MUNCKWIKLAND E; PAVELIC K; DACIC S; DANILOVIC Z; PAVELIC L; MUGGE RE; WILSON K; NGUYEN C; GLUCKMAN JL;
Indirizzi:
UNIV CINCINNATI,COLL MED,DEPT OTOLARYNGOL HEAD & NECK SURG,DIV MOLEC ONCOL CINCINNATI OH 45267 UNIV CINCINNATI,COLL MED,DEPT CELL BIOL NEUROBIOL & ANAT CINCINNATI OH 45267 KAROLINSKA HOSP,DEPT OTORHINOLARYNGOL STOCKHOLM SWEDEN RUDJER BOSKOVIC INST,DEPT MOLEC MED ZAGREB CROATIA UNIV ZAGREB,SCH MED,DEPT PATHOL ZAGREB CROATIA
Titolo Testata:
Anticancer research
fascicolo: 5B, volume: 14, anno: 1994,
pagine: 2259 - 2266
SICI:
0250-7005(1994)14:5B<2259:OOPPIC>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
SQUAMOUS-CELL CARCINOMA; TUMOR-SUPPRESSOR GENE; HUMAN MALIGNANCIES; COLORECTAL-CANCER; EMBEDDED TISSUES; HUMAN SARCOMAS; BREAST-CANCER; LATE EVENT; MUTATIONS; EXPRESSION;
Keywords:
HEAD AND NECK LESIONS; P53; IMMUNOHISTOCHEMISTRY; DNA SEQUENCING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
56
Recensione:
Indirizzi per estratti:
Citazione:
Z.P. Pavelic et al., "OVEREXPRESSION OF P53 PROTEIN IS COMMON IN PREMALIGNANT HEAD AND NECKLESIONS", Anticancer research, 14(5B), 1994, pp. 2259-2266

Abstract

In order to determine whether or not the p53 gene is involved in the malignant transformation of the head and neck carcinoma HNSCC, we haveanalyzed archival specimens from 527 primary head and neck lesions and 27 corresponding lymph node metastases. Nuclear p53 protein was present in 107 of 190 (56%) dysplasias, 61 of 102 (60%) carcinoma in situ (CIS), and 262 of 493 (53%) carcinomas. The p53 score did not increasesignificantly with progression of these lesions from dysplasia to CISand to carcinoma. All 357 normal samples of head and neck tissues were negative. The majority of the 172 sets of premalignant and malignantlesions displayed concordant p53 staining patterns. The staining was incongruous in only six cases. The p53 staining results were congruentin all 27 pairs of primary and metastatic (lymph nodes) tumors. Thesedata strongly suggest that p53 protein could be altered in a very early phase of the head and neck tumorigenesis and is maintained during tumor progression and metastatic spread. Mutations in p53 were examinedin 11 cases that exhibited high levels of p53 protein as detected by immunohistochemistry using PAb 1801 MAb. Mutation analysis was performed by direct sequencing of the PCR amplification products of exons 5 through 8, which contain greater than 90% of p53 mutations found in tumors. Three of 11 HNSCC had mutations at codon 130 (C to A), 193 (A to T), 283 (G to C), respectively. No mutations were found in the other 8samples within the regions examined. However, they may have mutationsin unsequenced regions of p53 or may have wild type protein that accumulates for other reasons.

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Documento generato il 02/04/20 alle ore 21:49:21