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Titolo:
VASCULAR ENDOTHELIAL GROWTH-FACTOR AND GLIOMA ANGIOGENESIS - COORDINATE INDUCTION OF VEGF RECEPTORS, DISTRIBUTION OF VEGF PROTEIN AND POSSIBLE IN-VIVO REGULATORY MECHANISMS
Autore:
PLATE KH; BREIER G; WEICH HA; MENNEL HD; RISAU W;
Indirizzi:
KLINIKUM PHILIPPS UNIV,NEUROPATHOL ABT,BALDINGERSTR 1 D-35043 MARBURGGERMANY MAX PLANCK INST PHYSIOL & CLIN RES,DEPT MOLEC CELL BIOL BAD NAUHEIM GERMANY GESELL BIOTECHNOL FORSCH MBH,DEPT GENE EXPRESS W-3300 BRAUNSCHWEIG GERMANY
Titolo Testata:
International journal of cancer
fascicolo: 4, volume: 59, anno: 1994,
pagine: 520 - 529
SICI:
0020-7136(1994)59:4<520:VEGAGA>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERMEABILITY FACTOR; TUMOR ANGIOGENESIS; TYROSINE KINASE; EXPRESSION; CELLS; GENE; PROGRESSION; INVIVO; MODEL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
K.H. Plate et al., "VASCULAR ENDOTHELIAL GROWTH-FACTOR AND GLIOMA ANGIOGENESIS - COORDINATE INDUCTION OF VEGF RECEPTORS, DISTRIBUTION OF VEGF PROTEIN AND POSSIBLE IN-VIVO REGULATORY MECHANISMS", International journal of cancer, 59(4), 1994, pp. 520-529

Abstract

We have previously suggested that tumor angiogenesis in human gliomasis regulated by a paracrine mechanism involving vascular endothelial growth factor (VEGF) and flt-1 (VEGF-receptor I), VEGF, an endothelial-cell-specific mitogen, is abundantly expressed in glioma cells which reside along necrotic areas, whereas flt-1, a tyrosine-kinase receptorfor VEGF, is expressed in tumor endothelial cells, but not in endothelial cells in normal adult brain. Recently, a second tyrosine-kinase receptor which binds VEGF with high affinity, designated KDR or flk-1, has been described, We performed in situ hybridization for VEGF mRNA, flt-1 mRNA and KDR mRNA on serial sections of normal brain, low-grade and high-grade glioma specimens, We show that KDR mRNA is co-expressedwith flt-1 in vascular cells in glioblastoma but not in low-grade glioma. Since fit-1 and KDR are not expressed in endothelial cells in thenormal adult brain, the coordinate up-regulation of 2 receptors for VEGF appears to be a critical event which controls tumor angiogenesis. Immunocytochemistry with a monoclonal anti-VEGF antibody revealed significant amounts of VEGF protein in the same glioma cells that expressed VEGF mRNA. The largest amount of VEGF immunoreactivity, however, wasdetected on the vasculature of glioblastomas, the site where VEGF exerts its biological functions. These findings suggest that VEGF is produced and secreted by glioma cells and acts on tumor endothelial cells which express VEGF receptors. To further characterize VEGF-producer cells in vivo, we investigated cellular proliferation, immunoreactivity to the p53 tumor-suppressor gene product and epidermal-growth-factor-receptor(EGFR) expression on serial sections by immunocytochemistry. VEGF-producer cells did not show increased cellular proliferation, p53 immunoreactivity or EGFR immunoreactivity as compared with glioma cellswhich did not express VEGF, Our studies therefore do not demonstrate evidence for a growth advantage of VEGF-producer cells in vivo or VEGFinduction by p53 mutation or EGFR over-expression. (C) 1994 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 16:52:08