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Titolo:
DIFFERENTIAL PLATELET-ASSOCIATED FACTOR GENE-EXPRESSION BY A PANEL OFHUMAN CELL-LINES WITH MEGAKARYOBLASTIC PROPERTIES AFTER TREATMENT WITH PHORBOL-MYRISTATE ACETATE
Autore:
MICALLEF M; MATSUO Y; TAKAYAMA K; ARIYASU T; OTANI T; MINOWADA J;
Indirizzi:
HAYASHIBARA BIOCHEM LABS INC,FUJISAKI INST,675-1 FUJISAKI OKAYAMA 702JAPAN HAYASHIBARA BIOCHEM LABS INC,FUJISAKI CELL CTR OKAYAMA 702 JAPAN
Titolo Testata:
Hematological oncology
fascicolo: 4, volume: 12, anno: 1994,
pagine: 163 - 174
SICI:
0278-0232(1994)12:4<163:DPFGBA>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEGAKARYOCYTIC DIFFERENTIATION; C-SIS; LEUKEMIA; INTERLEUKIN-6; GLYCOPROTEINS; PATHOGENESIS; ALPHA;
Keywords:
MEGAKARYOCYTES; PMA; GENE EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
M. Micallef et al., "DIFFERENTIAL PLATELET-ASSOCIATED FACTOR GENE-EXPRESSION BY A PANEL OFHUMAN CELL-LINES WITH MEGAKARYOBLASTIC PROPERTIES AFTER TREATMENT WITH PHORBOL-MYRISTATE ACETATE", Hematological oncology, 12(4), 1994, pp. 163-174

Abstract

Four human megakaryocytoid cell lines, namely MOLM-1, MOLM-7, MEG-01 and HEL, were treated with phorbol 12-myristate 13-acetate (PMA) and expression of the platelet-derived growth factor (PDGF) A chain, von Willebrand factor (vWF) and endothelial cell growth factor (ECGF) genes was examined by the reverse transcriptase-polymerase chain reaction (RT-PCR) using specific primers. The gene for PDGF A chain is constitutively weakly expressed by MEG-01 cells and strong expression is inducedin MEG-01, MOLM-1 and MOLM-7 but not in HEL cells after treatment with PMA for three days. All four cell lines express the VWF gene both constitutively and after exposure to PMA. None of the cell lines constitutively express the gene for ECGF but MEG-01 cells can be induced to do so after treatment with the phorbol diester. Immunohistochemical staining after exposure to PMA showed that the expression of the platelet-associated markers CD41 and CD61 was enhanced in all cell lines indicating possible differentiation along the megakaryocyte lineage. Our results illustrate differential platelet-associated factor gene expression in different megakaryoblastic cell populations in response to treatment with PMA, and suggest that expressions of the PDGF A chain gene and the ECGF gene may be good markers for megakaryocyte maturation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 05:14:25