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Titolo:
OXIDANTS AFFECT PERMEABILITY AND REPAIR OF THE CULTURED HUMAN TRACHEAL EPITHELIUM
Autore:
YAMAYA M; SEKIZAWA K; MASUDA T; MORIKAWA M; SAWAI T; SASAKI H;
Indirizzi:
TOHOKU UNIV,SCH MED,DEPT GERIATR MED,AOBA KU,1-1 SEIRYO MACHI SENDAI MIYAGI JAPAN TOHOKU UNIV,SCH MED,DEPT GERIATR MED,AOBA KU SENDAI MIYAGI JAPAN TOHOKU UNIV,SCH MED,DEPT PATHOL SENDAI MIYAGI 980 JAPAN
Titolo Testata:
American journal of physiology. Lung cellular and molecular physiology
fascicolo: 2, volume: 12, anno: 1995,
pagine: 284 - 293
SICI:
1040-0605(1995)12:2<284:OAPARO>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
RESPIRATORY EPITHELIUM; STRAND SCISSION; OXYGEN RADICALS; INJURY; SUPEROXIDE; EXPOSURE; CONDUCTANCE; MONOLAYERS; IONOPHORES; INVITRO;
Keywords:
OXYGEN RADICAL; EPITHELIAL PERMEABILITY; TIGHT JUNCTIONS; BRONCHIAL ASTHMA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
M. Yamaya et al., "OXIDANTS AFFECT PERMEABILITY AND REPAIR OF THE CULTURED HUMAN TRACHEAL EPITHELIUM", American journal of physiology. Lung cellular and molecular physiology, 12(2), 1995, pp. 284-293

Abstract

To examine the effects of oxidants on the airway epithelial barrier functions, human tracheal epithelial cells were cultured on porous filter membrane. Glucose oxidase (GO; 10 U/ml), hydrogen peroxide (H2O2; 4x 10(-3) M), and xanthine (5 x 10(-4) M) plus xanthine oxidase (20 mU/ml) (X-XO) significantly increased electrical conductance across epithelial membrane (G), short-circuit current (I-sc) measured with Ussing's chamber methods, and [H-3]mannitol flux through the cultured epithelium. Increases in G and I-sc induced by oxidants were significantly inhibited by catalase (1,000 U/ml) and the protein kinase C inhibitor staurosporine (10(-7) M), but superoxide dismutase (SOD; 100 U/ml) was without effect. GO, H2O2, and X-XO inhibited the epithelial cell growth, [H-3]thymidine incorporation by the cells, and epithelial repair ofartificially produced focal epithelial defects (1-2 mm diam) on plastic vessels. Catalase also inhibited effects induced by oxidants on cell growth and proliferation. These results suggest that oxidants reducetracheal epithelial barrier functions by damaging tight junctions andinhibiting cell proliferation, and these effects of oxidants on epithelial cells may be mediated by H2O2 rather than superoxide anion and by activation of protein kinase C.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/08/20 alle ore 08:57:57