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Titolo:
HYDRODYNAMIC AND ELECTRICAL CHARACTERIZATION OF T-VIMENTIN DIMERS ANDTETRAMERS BY TRANSIENT ELECTRIC BIREFRINGENCE MEASUREMENTS
Autore:
KOOIJMAN M; BLOEMENDAL M; TRAUB P; VANGRONDELLE R; VANAMERONGEN H;
Indirizzi:
FREE UNIV AMSTERDAM,DEPT BIOPHYS,DE BOELELAAN 1081 1081 HV AMSTERDAM NETHERLANDS ROYAL FREE HOSP,SCH MED,DEPT PROT & MOLEC BIOL LONDON NW3 2PF ENGLAND MAX PLANCK INST CELL BIOL D-68526 LADENBURG GERMANY
Titolo Testata:
The Journal of biological chemistry
fascicolo: 7, volume: 270, anno: 1995,
pagine: 2931 - 2937
SICI:
0021-9258(1995)270:7<2931:HAECOT>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
COILED-COIL MOLECULES; 1/KERATIN-10 INTERMEDIATE FILAMENTS; FIBRILLARY ACIDIC PROTEIN; INTEGRAL-EQUATIONS; DESMIN; INVITRO; POLYMORPHISM; REGISTER; MYOSIN; CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
M. Kooijman et al., "HYDRODYNAMIC AND ELECTRICAL CHARACTERIZATION OF T-VIMENTIN DIMERS ANDTETRAMERS BY TRANSIENT ELECTRIC BIREFRINGENCE MEASUREMENTS", The Journal of biological chemistry, 270(7), 1995, pp. 2931-2937

Abstract

The structure and charge distribution of T-vimentin, which differs from the intact intermediate filament protein vimentin through the absence of the first 70 amino acids, has been studied by transient electricbirefringence measurements. It is found that in 0.7 mM phosphate, pH 7.5 buffer, exclusively single dimers (with a hydrodynamic length of 40 to 43 nm) are present, which are considerably bent and/or flexible and which have a relatively large permanent dipole moment. This indicates a parallel alignment of two protein chains. In 0.2 mM phosphate, 0.5 mM MgCl2, pH 7.5, predominantly tetrameric T-vimentin is found with a rigid structure, no permanent dipole moment, and a length of 63 to 68 nm. Tetramer formation is likely to be induced by binding of Mg2+ tothe protein. The observed length is in agreement with that of intact vimentin tetramers in which the 1B regions of the rod domains of the dimers overlap (A(11) configuration). A minor part of the tetramers maybe in a flexible or bent A(22) form. The loss of the permanent dipolemoment when tetramers are formed is, apart from charge compensation, presumably due to the antiparallel alignment of the constituting dimers in which their dipoles cancel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 00:13:56