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Titolo:
IN-VITRO AND IN-VIVO ANALYSIS OF MURINE LIPOPROTEIN-LIPASE GENE PROMOTER - TISSUE-SPECIFIC EXPRESSION
Autore:
GIMBLE JM; HUA XX; WANKER F; MORGAN C; ROBINSON C; HILL MR; NADON N;
Indirizzi:
OKLAHOMA MED RES FDN,IMMUNOBIOL & CANC PROGRAM,825 NE 13TH ST OKLAHOMA CITY OK 73104 UNIV OKLAHOMA,OKLAHOMA CTR MOLEC MED OKLAHOMA CITY OK 73190 UNIV OKLAHOMA,DEPT CLIN SCI OKLAHOMA CITY OK 73190 UNIV OKLAHOMA,DEPT PATHOL OKLAHOMA CITY OK 73190 UNIV OKLAHOMA,DEPT ZOOL OKLAHOMA CITY OK 73190 OKLAHOMA CHRISTIAN UNIV SCI ARTS,DEPT NAT SCI OKLAHOMA CITY OK 73136 UNIV TULSA,DEPT BIOL TULSA OK 74104
Titolo Testata:
American journal of physiology: endocrinology and metabolism
fascicolo: 2, volume: 31, anno: 1995,
pagine: 213 - 218
SICI:
0193-1849(1995)31:2<213:IAIAOM>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA; TRANSGENIC MICE; TRANSCRIPTION; EVOLUTION; REGION; CELLS; SITE;
Keywords:
LUCIFERASE; OCTAMER; TRANSCRIPTION; TRANSGENIC MICE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
J.M. Gimble et al., "IN-VITRO AND IN-VIVO ANALYSIS OF MURINE LIPOPROTEIN-LIPASE GENE PROMOTER - TISSUE-SPECIFIC EXPRESSION", American journal of physiology: endocrinology and metabolism, 31(2), 1995, pp. 213-218

Abstract

Lipoprotein lipase, an enzyme of central importance to lipid metabolism, is most abundant in adipose tissues, cardiac and skeletal muscle, and portions of the brain. The current work examined the murine lipoprotein lipase promoter using transient transfection, gel-retention analyses, and transgenic mice. Maximum expression of the luciferase reporter gene in transfected cells was observed with -101 bp of the promoter. Nuclear extracts from tissues expressing lipoprotein lipase contained DNA binding proteins that recognize the CCAAT box (-64 bp) and an octamer motif (-46 bp); this combination of factors was absent in nonexpressing tissues. Transgenic mice from three of five founders prepared with -1,824-bp promoter constructs expressed the luciferase reporter gene at highest levels in brown adipose tissue and brain. These findings suggest that the -1,824-bp promoter region contains sequence elements responsible for the tissue-specific transcription of lipoprotein lipase in vivo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 03:56:11