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Titolo:
HUMAN CYTOMEGALOVIRUS UL102 GENE
Autore:
SMITH JA; PARI GS;
Indirizzi:
HYBRIDON INC,1 INNOVAT DR WORCESTER MA 01605 HYBRIDON INC WORCESTER MA 01605
Titolo Testata:
Journal of virology
fascicolo: 3, volume: 69, anno: 1995,
pagine: 1734 - 1740
SICI:
0022-538X(1995)69:3<1734:HCUG>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
SIMPLEX VIRUS TYPE-1; DEPENDENT DNA-SYNTHESIS; HELICASE-PRIMASE; TRANSIENT COMPLEMENTATION; POLYMERASE GENE; EARLY PROMOTER; PRODUCTS; SEQUENCE; TRANSCRIPTION; REPLICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
J.A. Smith e G.S. Pari, "HUMAN CYTOMEGALOVIRUS UL102 GENE", Journal of virology, 69(3), 1995, pp. 1734-1740

Abstract

We have identified and characterized the transcript for the human cytomegalovirus (HCMV) UL102 gene. The UL102 gene product is proposed to encode the primase-associated factor. The primase-associated factor isone of the three components of the helicase-primase complex, along with UL105 (helicase) and UL70 (primase). In order to characterize the UL102 transcription unit we used single-stranded antisense RNA probes to identify an abundant 2.7-kb transcript originating from the UL102 region. This transcript can be initially detected at 24 h postinfection and in the presence of phosphonoformic acid but not in the presence ofcycloheximide. A 2.7-kb cDNA clone containing this transcript was isolated from a 72-h HCMV (strain Towne) cDNA library. Sequence analysis of this clone revealed a continuous unspliced transcript between the region of UL101X and UL102; the only in-frame translational stop codon is 2,619 bp downstream from the first ATG in the message. Genome sequencing of the UL102 region from strains AD169 and Towne revealed that the UL101X stop codon TAA was actually TAC and that the cDNA and genomic sequences were in agreement. The cDNA clone starts 5 nucleotides (nt) upstream of the UL101X ATG, continues through the putative ATG of UL102, and ends 97 nt downstream of the putative termination codon of the UL102 open reading frame. Primer extension analysis indicated a transcriptional start site 23 nt upstream of the UL1O1X open reading frame.

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Documento generato il 27/11/20 alle ore 13:39:29