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Titolo:
EVIDENCE FOR A REGULATORY ELEMENT CONTROLLING AMINO-ACID-TRANSPORT SYSTEM-L IN CHINESE-HAMSTER OVARY CELLS
Autore:
COLLARINI EJ; CAMPBELL GS; OXENDER DL;
Indirizzi:
UNIV LONDON UNIV COLL,MOLEC CELL BIOL LAB,GOWER ST LONDON WC1E 6BT ENGLAND UNIV MICHIGAN,DEPT BIOL CHEM ANN ARBOR MI 48109
Titolo Testata:
Journal of cellular biochemistry
fascicolo: 4, volume: 56, anno: 1994,
pagine: 544 - 549
SICI:
0730-2312(1994)56:4<544:EFAREC>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSFER RNA-SYNTHETASE; EXPRESSION CLONING; LEUCINE TRANSPORT; RAT-KIDNEY; CLUSTER; CDNA;
Keywords:
AMINO ACID TRANSPORT; TRANSPORT REGULATION; TRANSPORT SYSTEM L; CHO CELLS; HYBRID CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
E.J. Collarini et al., "EVIDENCE FOR A REGULATORY ELEMENT CONTROLLING AMINO-ACID-TRANSPORT SYSTEM-L IN CHINESE-HAMSTER OVARY CELLS", Journal of cellular biochemistry, 56(4), 1994, pp. 544-549

Abstract

We have used the technique of somatic cell hybridization to study theregulation of the neutral amino acid transport system L in Chinese hamster ovary (CHO) cells. The cell line CHO-ts025C1 has a temperature-sensitive mutation in leucyl-tRNA synthetase. At the nonpermissive temperature of 39 degrees C, CHO-ts025C1 cells are unable to charge leucyl-tRNA and behave as though starved for leucine by increasing their system L transport activity two- to fourfold. From the temperature-sensitive cell line, we have isolated a regulatory mutant cell line, CHO-C11B6, that has constitutively elevated system L transport activity. The CHO-C11B6 cell line retains the temperature-sensitive leucyl-tRNA synthetase mutation, but growth of this cell line is temperature resistantbecause its increased system L transport activity leads to increased intracellular leucine levels, which compensate for the defective synthetase. Hybrid cells formed by fusion of the temperature-sensitive CHO-ts025C1 cells and the temperature-resistant CHO-C11B6 cells show temperature-sensitive growth and temperature-dependent regulation of leucine transport activity. These data suggest that the system L activity ofCHO cells is regulated by a dominant-acting element that is defectiveor absent in the regulatory mutant CHO-C11B6 cell line. (C) 1994 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/01/21 alle ore 07:32:42