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Titolo:
BINDING IN-VITRO OF PHOSPHOTYROSINE-CONTAINING PROTEINS TO PP60(C-SRC) SH2 DOMAIN DOES NOT CORRELATE WITH CEF CELL-TRANSFORMATION
Autore:
BOEUF H; MURPHY J; BIBBINS KB; VARMUS HE;
Indirizzi:
IGBMC,BP 163 F-67404 ILLKIRCH GRAFFENS FRANCE CU STRASBOURG FRANCE CHIRON CORP,DEPT VIROL EMERYVILLE CA 94608 UNIV CALIF SAN FRANCISCO,DEPT MICROBIOL & IMMUNOL SAN FRANCISCO CA 94143 NIH BETHESDA MD 20892
Titolo Testata:
Oncogene
fascicolo: 3, volume: 10, anno: 1995,
pagine: 433 - 438
SICI:
0950-9232(1995)10:3<433:BIOPPT>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
GTPASE-ACTIVATING PROTEIN; GROWTH-FACTOR RECEPTORS; SRC HOMOLOGY REGION-2; C-SRC; V-SRC; SIGNAL TRANSDUCTION; PP60(SRC) SUBSTRATE; DEPENDENT MANNER; TYROSINE KINASE; HIGH-AFFINITY;
Keywords:
SH2; SH3; SRC; TRANSFORMATION; PHOSPHOTYROSINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
57
Recensione:
Indirizzi per estratti:
Citazione:
H. Boeuf et al., "BINDING IN-VITRO OF PHOSPHOTYROSINE-CONTAINING PROTEINS TO PP60(C-SRC) SH2 DOMAIN DOES NOT CORRELATE WITH CEF CELL-TRANSFORMATION", Oncogene, 10(3), 1995, pp. 433-438

Abstract

We have previously described pp60(c-src) SH2 mutants that are host-range-dependent for cell transformation; most of these mutants can transform CEF cells but not NIH3T3 cells, and others transform NM3T3 cells more efficiently than CEF (Hirai and Varmus, 1990c). In an attempt to understand the molecular basis of these phenotypes, we analysed the ability of mutant SH2 domains in GST fusion-proteins to bind to tyrosinephosphorylated proteins in lysates from CEF and NIH3T3 cells. The relative affinity of mutated versions of the SH2 domain for phosphotyrosine-containing proteins from CEF and NM3T3 cells was compared with the relative ability of the mutant Src proteins to transform the two cell types. While the affinity of the SH2 domain for phosphotyrosine-containing proteins was closely correlated with transformation in NIH3T3 cells, there was no correlation between phosphotyrosine binding and transformation of CEF cells, and none of the host range mutant SH2 domains showed significant differences in their ability to bind phosphotyrosine-containing proteins from lysates from either cell type. In addition,the SH3 domain was shown to augment the capacity of mutant SH2 domainto bind phosphotyrosine-containing proteins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 09:24:21