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Titolo:
CHANGES IN THE URINARY-EXCRETION LEVEL OF 8-HYDROXYGUANINE BY EXPOSURE TO REACTIVE OXYGEN-GENERATING SUBSTANCES
Autore:
SUZUKI J; INOUE Y; SUZUKI S;
Indirizzi:
SCI UNIV TOKYO,FAC PHARMACEUT SCI,SHINJUKU KU,12 ICHIGAYA TOKYO 162 JAPAN
Titolo Testata:
Free radical biology & medicine
fascicolo: 3, volume: 18, anno: 1995,
pagine: 431 - 436
SICI:
0891-5849(1995)18:3<431:CITULO>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
OXIDATIVE DNA DAMAGE; ENDONUCLEASE ACTIVITY; RADICALS; TOXICITY; 8-HYDROXY-2'-DEOXYGUANOSINE; SUPEROXIDE; PARTICLES; MECHANISM; RESIDUES; CANCER;
Keywords:
URINARY EXCRETION; 8-HYDROXYGUANINE; HUMAN URINE; RAT URINE; REACTIVE OXYGEN; 2-NITROPROPANE; SMOKING; DIESEL EXHAUST; FREE RADICALS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
J. Suzuki et al., "CHANGES IN THE URINARY-EXCRETION LEVEL OF 8-HYDROXYGUANINE BY EXPOSURE TO REACTIVE OXYGEN-GENERATING SUBSTANCES", Free radical biology & medicine, 18(3), 1995, pp. 431-436

Abstract

A simple means of measuring of 8-hydroxyguanine (8-OHGua) levels in urine was developed. Rat and human urine samples were purified by meansof strong cation exchange chromatography (Amberlite CG-120), followedby cellulose partition chromatography (Whatman CF-11). Thereafter, 8-OHGua was determined by means of high performance liquid chromatography (HPLC) using an electro-chemical detector. The level of 8-OHGua in rat urine increased by a factor of 2 to 4 after an intraperitoneal administration of 2-nitropropane (25 mg/kg), paraquat (11.3 mg/kg), or hydroquinone (11 mg/kg). On the other hand, the urine of smokers and persons exposed to air polluted with car exhaust also contained 1.9 and 3.8 fold more 8-OHGua, respectively, than that of control nonsmokers. These results indicated that the amount of 8-OHGua in urine is useful marker for monitoring the level of in vivo oxidative stress.

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Documento generato il 12/07/20 alle ore 12:33:40