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Titolo:
TRANSPLANTATION OF ALLOGENEIC HEPATOCYTES WITHOUT IMMUNOSUPPRESSION -LONG-TERM SURVIVAL
Autore:
BALLADUR P; CREMA E; HONIGER J; CALMUS Y; BAUDRIMONT M; DELELO R; CAPEAU J; NORDLINGER B;
Indirizzi:
HOP ST ANTOINE,CTR CHIRURG DIGEST,184 RUE FAUBOURG ST ANTOINE F-75012PARIS FRANCE HOP ST ANTOINE,DEPT PATHOL F-75012 PARIS FRANCE HOP ST ANTOINE,DEPT SURG F-75012 PARIS FRANCE HOP ST ANTOINE,INSERM,RES UNIT 402 F-75012 PARIS FRANCE
Titolo Testata:
Surgery
fascicolo: 2, volume: 117, anno: 1995,
pagine: 189 - 194
SICI:
0039-6060(1995)117:2<189:TOAHWI>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
MICROCARRIER-ATTACHED HEPATOCYTES; RAT HEPATOCYTES; LIVER-FUNCTION; SUPPORT; CELLS; GUNN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
P. Balladur et al., "TRANSPLANTATION OF ALLOGENEIC HEPATOCYTES WITHOUT IMMUNOSUPPRESSION -LONG-TERM SURVIVAL", Surgery, 117(2), 1995, pp. 189-194

Abstract

Background. Hepatocyte transplantation could be an alternative to whole liver transplantation. Allogeneic hepatocytes are rejected if transplanted without immunosuppression. The aim of this study was to transplant allogeneic hepatocytes in the peritoneum and to protect them fromrejection by encapsulation in a new semipermeable membrane. Methods. Rat hepatocytes were encapsulated in hydrogel-based hollow fibers, obtained from AN69 copolymer, before being transplanted into the peritoneum of rats. Outcome of allogeneic hepatocytes encapsulated in hollow fibers was compared with that of syngeneic hepatocytes encapsulated in hollow fibers, with that of free allogeneic hepatocytes, and with allogeneic hepatocytes encapsulated in hollow fibers left open. Cell viability was assessed by erythrosin exclusion, structure by electron microscopy, and function by albumin release. Results. Up to 90 days, viability of allogeneic hepatocytes in hollow fibers was greater than 80%. The structure remained normal at electron microscopy. Albumin release was 16.5 +/- 0.3 mu g/24 hr/10(6) hepatocytes (day 15), 14.2 +/- 2.0 mug/24 hr/106 hepatocytes (day 30), 8.8 +/- 0.1 mu g/24 hr/10(6) hepatocytes (day 60), and 11.4 +/- 0.3 mu g/24 hr/10(6) hepatocytes (day 90). Free hepatocytes and hepatocytes in hollow fibers left open did not survive at day 15.Conclusions. Viability and function of encapsulated allogeneic hepatocytes were maintained up to 90 days after transplantation, without immunosuppression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/21 alle ore 14:55:36