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Titolo:
APPLICATION OF SOLID-STATE CATALYTIC HYDROGEN ISOTOPE-EXCHANGE TO THETRITIUM LABELING OF LYSOZYME
Autore:
FILIKOV AV; JONES JR; MYASOEDOV NF; WARD ES;
Indirizzi:
UNIV SURREY,DEPT CHEM GUILDFORD GU2 5XH SURREY ENGLAND RUSSIAN ACAD SCI,INST MOLEC GENET MOSCOW 123182 RUSSIA UNIV TEXAS,SW MED CTR,CTR CANC IMMUNOBIOL DALLAS TX 75235 UNIV TEXAS,SW MED CTR,DEPT MICROBIOL DALLAS TX 75235
Titolo Testata:
Journal of labelled compounds & radiopharmaceuticals
fascicolo: 2, volume: 36, anno: 1995,
pagine: 179 - 185
SICI:
0362-4803(1995)36:2<179:AOSCHI>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
ORGANIC-COMPOUNDS; KINETICS;
Keywords:
ISOTOPE EXCHANGE; PROTEINS; SOLID STATE HYDROGENATION; TRITIUM; DEUTERIUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
17
Recensione:
Indirizzi per estratti:
Citazione:
A.V. Filikov et al., "APPLICATION OF SOLID-STATE CATALYTIC HYDROGEN ISOTOPE-EXCHANGE TO THETRITIUM LABELING OF LYSOZYME", Journal of labelled compounds & radiopharmaceuticals, 36(2), 1995, pp. 179-185

Abstract

Solid state catalytic hydrogen isotope exchange has been employed to label hen egg lysozyme with tritium. Optimization of reaction conditions so that amino acids and peptide bonds remained intact led to a tritiated product with 97% of the original enzymatic activity and 94% radiochemical purity. The specific activity when using a T-2:H-2 mixture of 1:1000, was 16 mCi.mmol-(1). It is suggested that the currently adopted approach may have wide applications for other proteins able to tolerate lyophilization conditions without loss of activity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 22:26:06