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Titolo:
COLLECTION OF OOCYTES FROM CATTLE VIA FOLLICULAR ASPIRATION AIDED BY ULTRASOUND WITH OR WITHOUT GONADOTROPIN PRETREATMENT AND IN DIFFERENT REPRODUCTIVE STAGES
Autore:
BUNGARTZ L; LUCASHAHN A; RATH D; NIEMANN H;
Indirizzi:
INST TIERZUCHT & TIERVERHALTEN,FAL MARIENSEE GERMANY INST TIERZUCHT & TIERVERHALTEN,FAL MARIENSEE GERMANY
Titolo Testata:
Theriogenology
fascicolo: 3, volume: 43, anno: 1995,
pagine: 667 - 675
SICI:
0093-691X(1995)43:3<667:COOFCV>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
INVITRO FERTILIZATION; BOVINE EMBRYOS; RETRIEVAL; PUNCTURE;
Keywords:
ULTRASOUND; FOLLICLE ASPIRATION; CUMULUS OOCYTE COMPLEXES; BOVINE; BLASTOCYSTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
L. Bungartz et al., "COLLECTION OF OOCYTES FROM CATTLE VIA FOLLICULAR ASPIRATION AIDED BY ULTRASOUND WITH OR WITHOUT GONADOTROPIN PRETREATMENT AND IN DIFFERENT REPRODUCTIVE STAGES", Theriogenology, 43(3), 1995, pp. 667-675

Abstract

Ultrasound-guided follicular aspiration was performed on 29 Holstein-Friesian cows/heifers twice weekly at 3- to 4-d intervals over a period of 2 consecutive estrous cycles (total 42 d). For visualization of the ovaries and guidance of the aspiration needle, a 6.5 MHz fingertip probe on a 62 cm probe carrier was inserted into the vagina. The disposable aspiration needle was connected to a permanent rinse tubing system, thus ensuring minimum death of oocytes in the aspiration processs. After penetration of the vaginal wall, the needle was inserted into afollicle of the rectally fixed ovary. Cumulus oocyte complexes (COG) were aspirated at a pressure of 100 mm Hg. In the first experiment, the effect of an additional gonadotropin treatment 4 d prior to aspiration was investigated in 8 lactating cows. Following FSH-treatment, the number of aspirated follicles was higher (P < 0.05) than in the nontreated animals (10.6 +/- 0.7 vs 8.9 +/- 0.5). The number of recovered COC (7.0 +/- 0.6 vs 5.8 +/- 0.5), the recovery rate (COG per aspirated follicle) (66.6% vs 65.4%), the percentage of viable COC (56.8% vs 52.1%), the cleavage rate upon in vitro maturation and in vitro fertilization (56.7% vs 59.8%) as well as the rate of morula/blastocyst formation (3.8% vs 2.9%) were similar in both groups. in the second experiment, follicles were aspirated in 4 lactating cows, 6 dry cows, 4 pregnantcows (first 35 d of pregnancy), and 4 heifers. The average number of aspirated follicles and recovered COC was higher (P < 0.05) in the first 2 groups (10.6 +/- 0.6 and 9.3 +/- 0.7 follicles; 7.2 +/- 0.5 and 6.9 +/- 0.7 oocytes) than in the 2 other treatment groups (7.3 +/- 0.5 and 8.1 +/- 0.5 follicles; 5.0 +/- 0.4 and 5.7 +/- 0.5 oocytes). The percentage of viable COC was higher (P < 0.05; 68.3%) in lactating animals than in all the other groups' (49.7, 52.5 and 57.4%, respectively). Similarly, upon invitro fertilization, cleavage rate was higher (P <0.05; 63.4%) in lactating cows than in the other groups (43.7, 50.5, 55.1% respectively). A total of 21.5, 22.7, 11.9 and 13.5%; respectively, in the 4 groups of the in vitro fertilized oocytes reached the morula and blastocyst stages. After transfer of a total of 48 embryos 22 pregnancies (45.8%) were established as detected on Day 65. We conclude that I) repeated aspiration of viable COC at short intervals is possible, 2) additional FSH-treatment does not increase oocyte yields, and3) viable blastocysts can be produced from cattle at various reproductive phases irrespective of the reproductive phase.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 14:44:43