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Titolo:
EVIDENCE FOR A NOVEL MITOCHONDRIAL PROMOTER PRECEDING THE COX2 GENE OF PERENNIAL TEOSINTES
Autore:
NEWTON KJ; WINBERG B; YAMATO K; LUPOLD S; STERN DB;
Indirizzi:
UNIV MISSOURI,DIV BIOL SCI COLUMBIA MO 65211 CORNELL UNIV,BOYCE THOMPSON INST PLANT RES ITHACA NY 14853
Titolo Testata:
EMBO journal
fascicolo: 3, volume: 14, anno: 1995,
pagine: 585 - 593
SICI:
0261-4189(1995)14:3<585:EFANMP>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSCRIPTION INITIATION SITES; CYTOPLASMIC MALE-STERILITY; CYTOCHROME-OXIDASE; MAIZE MITOCHONDRIA; SEQUENCE; GENOME; RNA; IDENTIFICATION; PLANT; ORGANIZATION;
Keywords:
COX2; MAIZE; MITOCHONDRIAL GENE; PROMOTER; TRANSCRIPTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
K.J. Newton et al., "EVIDENCE FOR A NOVEL MITOCHONDRIAL PROMOTER PRECEDING THE COX2 GENE OF PERENNIAL TEOSINTES", EMBO journal, 14(3), 1995, pp. 585-593

Abstract

We have characterized two promoters of the cytochrome oxidase subunit2 (cox2) gene in Zea perennis mitochondria present in maize lines. Initiation at a site 907 bases upstream of the start codon results in the major similar to 1900 nt cox2 transcript. A sequence just upstream of this site conforms to the consensus described for maize mitochondrial promoters and its transcription is correctly initiated in a maize mitochondrial in vitro transcription extract. A second transcription initiation site (-347) is used only when the dominant allele of a nucleargene, Mct, is present and its use results in an additional, shorter major transcript, Sequences flanking the Mct-dependent transcription initiation site, which we have termed the conditional promoter of cox2 (cpc), do not fit the maize mitochondrial promoter consensus and do notfunction in the maize in vitro transcription extract. The cpc region does not hybridize with mitochondrial, chloroplast or nuclear DNAs from most maize or teosinte lines, However, the cpc sequence is found in the same position upstream of the cox2 gene in Zea diploperennis mtDNAand it has striking similarity to the previously reported 'ORF of unknown origin' fused to the ATPase subunit 6 gene in maize CMS-C mitochondria, cpc appears to represent a new type of mitochondrial promoter, Further analysis of both conditional and constitutive promoters shouldhelp us to better understand the control of transcription in plant mitochondria.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 07:03:49