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Titolo:
THE GAMMA-2 SUBUNIT OF THE GABA(A) RECEPTOR IS CONCENTRATED IN SYNAPTIC JUNCTIONS CONTAINING THE ALPHA-1 AND BETA-2 3 SUBUNITS IN HIPPOCAMPUS, CEREBELLUM AND GLOBUS-PALLIDUS/
Autore:
SOMOGYI P; FRITSCHY JM; BENKE D; ROBERTS JDB; SIEGHART W;
Indirizzi:
MRC,DEPT PHARMACOL,ANAT NEUROPHARMACOL UNIT,MANSFIELD RD OXFORD OX1 3TH ENGLAND UNIV ZURICH,INST PHARMACOL CH-8057 ZURICH SWITZERLAND UNIV VIENNA,PSYCHIAT CLIN,DEPT BIOCHEM PSYCHIAT A-1090 VIENNA AUSTRIA
Titolo Testata:
Neuropharmacology
fascicolo: 9-10, volume: 35, anno: 1996,
pagine: 1425 - 1444
SICI:
0028-3908(1996)35:9-10<1425:TGSOTG>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADULT-RAT BRAIN; A-RECEPTOR; AMINOBUTYRIC-ACID; MONOCLONAL-ANTIBODIES; BENZODIAZEPINE RECEPTORS; GABA(A)-RECEPTOR SUBTYPES; IMMUNOCYTOCHEMICAL LOCALIZATION; SUBCELLULAR-LOCALIZATION; CHANNEL COMPLEX; PYRAMIDAL CELLS;
Keywords:
GABA(A) RECEPTOR; SUBUNIT COMPOSITION; BENZODIAZEPINE RECEPTOR; ELECTRON MICROSCOPY; IMMUNOCYTOCHEMISTRY; HIPPOCAMPUS; PYRAMIDAL CELL; CEREBELLUM; GLOBUS PALLIDUS; INHIBITION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
69
Recensione:
Indirizzi per estratti:
Citazione:
P. Somogyi et al., "THE GAMMA-2 SUBUNIT OF THE GABA(A) RECEPTOR IS CONCENTRATED IN SYNAPTIC JUNCTIONS CONTAINING THE ALPHA-1 AND BETA-2 3 SUBUNITS IN HIPPOCAMPUS, CEREBELLUM AND GLOBUS-PALLIDUS/", Neuropharmacology, 35(9-10), 1996, pp. 1425-1444

Abstract

The gamma 2 subunit is necessary for the expression of the full benzodiazepine pharmacology of GABA(A) receptors and is one of the major subunits in the brain. In order to determine the location of channels containing the gamma 2 subunit in relation to GABA-releasing terminals on the surface of neurons, a new polyclonal antipeptide antiserum was developed to the gamma 2 subunit and used in high resolution, postembedding, immunoelectron-microscopic procedures. Dual immunogold labellingof the same section for two subunits, and up to three sections of thesame synapse reacted for different subunits, were used to characterize the subunit composition of synaptic receptors. The gamma 2 subunit was present in type 2, ''symmetrical'' synapses in each of the brain areas studied, with the exception of the granule cell layer of the cerebellum. The gamma 2 subunit was frequently co-localized in the same synaptic junction with the alpha 1 and beta 2/3 subunits. The immunolabelling of synapses was coincident with the junctional membrane specialization of the active zone. Immunolabelling for the receptor often occurred in multiple clusters in the synapses. In the hippocampus, the gamma 2 subunit was present in basket cell synapses on the somata and proximal dendrites and in axo-axonic cell synapses on the axon initial segment of pyramidal and granule cells. Some synapses on the dendrites ofGABAergic interneurones were densely labelled for the gamma 2, alpha 1 and beta 2/3 subunits. In the cerebellum, the gamma 2 subunit was present in both distal and proximal Purkinje cell dendritic synapses established by stellate and basket cells, respectively. On the soma of Purkinje cells, basket cell synapses were only weakly labelled. Synapseson interneuron dendrites were more densely labelled for the gamma 2, alpha 1 and beta 2/3 subunits than synapses on Purkinje or granule cells. Although immunoperoxidase and immunofluorescence methods show an abundance of the gamma 2 subunit in granule cells, the labelling of Golgi synapses was much weaker with the immunogold method than that of the other cell types. In the globus pallidus, many type 2 synapses were labelled for the gamma 2 subunit together with alpha 1 and beta 2/3 subunits. The results show that gamma 2 subunit-containing receptor channels are highly concentrated in GABAergic synapses that also contain the alpha 1 and beta 2/3 subunits. Channels containing the gamma 2 subunit are expressed in synapses on functionally distinct domains of the same neuron receiving GABA from different presynaptic sources. There are quantitative differences in the density of GABA(A) receptors at synapses on different cell types in the same brain area. Copyright (C) 1996 Elsevier Science Ltd.

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Documento generato il 30/03/20 alle ore 09:08:17