Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
GABA(A) RECEPTOR SUBTYPES DIFFERENTIATED BY THEIR GAMMA-SUBUNIT VARIANTS - PREVALENCE, PHARMACOLOGY AND SUBUNIT ARCHITECTURE
Autore:
BENKE D; HONER M; MICHEL C; MOHLER H;
Indirizzi:
ETH ZURICH,INST PHARMACOL,WINTERTHURERSTR 190 CH-8057 ZURICH SWITZERLAND ETH ZURICH,INST PHARMACOL CH-8057 ZURICH SWITZERLAND UNIV ZURICH CH-8057 ZURICH SWITZERLAND
Titolo Testata:
Neuropharmacology
fascicolo: 9-10, volume: 35, anno: 1996,
pagine: 1413 - 1423
SICI:
0028-3908(1996)35:9-10<1413:GRSDBT>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMINOBUTYRIC ACIDA-RECEPTOR; DRUG-BINDING PROFILE; A-RECEPTOR; RAT-BRAIN; BENZODIAZEPINE RECEPTOR; ALPHA-SUBUNIT; GABA(A)/BENZODIAZEPINE RECEPTORS; IMMUNOAFFINITY PURIFICATION; MONOCLONAL-ANTIBODIES; DELTA-SUBUNIT;
Keywords:
GABA(A) RECEPTOR SUBTYPES; GAMMA-SUBUNITS; SUBUNIT-SPECIFIC ANTISERA; BENZODIAZEPINE RECEPTOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
54
Recensione:
Indirizzi per estratti:
Citazione:
D. Benke et al., "GABA(A) RECEPTOR SUBTYPES DIFFERENTIATED BY THEIR GAMMA-SUBUNIT VARIANTS - PREVALENCE, PHARMACOLOGY AND SUBUNIT ARCHITECTURE", Neuropharmacology, 35(9-10), 1996, pp. 1413-1423

Abstract

Native GABA(A) receptors containing different gamma-subunit variants were distinguished immunobiochemically with antisera selectively recognizing the gamma 1-, gamma 2- and gamma 3-subunits. While GABA(A) receptors containing the gamma 2-subunit were confirmed to be rather ubiquitous in the adult brain, receptors characterized by the gamma 1- or gamma 3-subunit were of low abundance, as shown by immunoprecipitation The three receptor populations differed strikingly in their benzodiazepine (BZ) site ligand binding profiles. The gamma 3-receptor population displayed reduced affinity for the full agonists clonazepam and flunitrazepam and virtually lacked sensitivity to zolpidem. The gamma 1-receptor population displayed low affinity for all benzodiazepine site ligands tested, except for flunitrazepam, and could be differentiated from the gamma 2- and gamma 3-receptors by its low affinity for the inverse agonist beta CCM and its lack of affinity for the partial inverseagonist Ro 15-4513 and the antagonist flumazenil. Since flumazenil antagonizes all major effects of BZ agonists, gamma 1-receptors are not involved in mediating these actions in vivo. In immunopurified receptors, the gamma-subunit variants were found to be assembled with different variants of alpha- and beta-subunits, indicating that not only the gamma 2-subunit but also the gamma 1- and gamma 3-subunits are part ofvarious receptor subtypes. In addition, the gamma 2- and gamma 3-subunits can be co-assembled in native receptors, consistent with the subunit stoichiometry of two alpha-, one beta- and two gamma-subunits proposed previously for recombinant receptors. Copyright (C) 1996 ElsevierScience Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 19:03:49