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Titolo:
CASCADE REGULATION OF TERMINAL ADIPOCYTE DIFFERENTIATION BY 3 MEMBERSOF THE C EBP FAMILY OF LEUCINE-ZIPPER PROTEINS/
Autore:
YEH WC; CAO ZD; CLASSON M; MCKNIGHT SL;
Indirizzi:
TULARIK INC S SAN FRANCISCO CA 94080 TULARIK INC S SAN FRANCISCO CA 94080 JOHNS HOPKINS UNIV,DEPT BIOL BALTIMORE MD 21218
Titolo Testata:
Genes & development
fascicolo: 2, volume: 9, anno: 1995,
pagine: 168 - 181
SICI:
0890-9369(1995)9:2<168:CROTAD>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENHANCER-BINDING-PROTEIN; TISSUE-SPECIFIC EXPRESSION; 3T3-L1 PREADIPOCYTES; ADIPOSE CONVERSION; NUCLEAR-PROTEIN; GENE-EXPRESSION; MESSENGER-RNAS; ALBUMIN GENE; CELLS; ALPHA;
Keywords:
ADIPOCYTE DIFFERENTIATION; CCAAT ENHANCER BINDING PROTEIN (C/EBP)ALPHA; C/EBP-BETA; C/EBP-DELTA; LIVER-ENRICHED TRANSCRIPTIONAL ACTIVATOR PROTEIN (LAP); LIVER-ENRICHED TRANSCRIPTIONAL INHIBITORY PROTEIN (LIP);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
57
Recensione:
Indirizzi per estratti:
Citazione:
W.C. Yeh et al., "CASCADE REGULATION OF TERMINAL ADIPOCYTE DIFFERENTIATION BY 3 MEMBERSOF THE C EBP FAMILY OF LEUCINE-ZIPPER PROTEINS/", Genes & development, 9(2), 1995, pp. 168-181

Abstract

Terminal differentiation of cultured 3T3-L1 fibroblasts to the adipogenic phenotype is potently stimulated by dexamethasone (DEX) and methylisobutylxanthine (MIX). Previous studies have shown that these hormones induce the expression of genes encoding two members of the CCAAT/enhancer binding protein (C/EBP) family of transcription factors. In theabsence of new protein synthesis DEX activates the gene encoding C/EBP delta. Likewise, MIX is a direct inducer of C/EBP beta gene expression. Optimal conditions for differentiation entail a 2-day period wherein confluent fibroblasts are exposed to DEX and MIX, followed by removal of the hormones and subsequent culture in the presence of insulin and fetal bovine serum. During the early phase of differentiation, highlevels of C/EBP delta and C/EBP beta accumulate. These transcription factors diminish during the terminal phase of differentiation and cometo be replaced by a third member of the C/EBP family, C/EBP alpha. Conclusive evidence has already shown that C/EBP alpha regulates terminal adipocyte differentiation, turning on the battery of fat-specific genes required for the synthesis, uptake, and storage of long chain fatty acids. Here we provide evidence that C/EBP delta and C/EBP beta playearly catalytic roles in the differentiation pathway, relaying the effects of the hormonal stimulants DEX and MIX in a cascade-like fashion, leading to the activation of the gene encoding C/EBP alpha. Conditions facilitating the precocious expression of either C/EBP delta or C/EBP beta were observed to accelerate adipogenesis and, in the case of C/EBP beta, relieve dependence on the early hormonal stimulants. Likewise, conditions that prevented the expression of functional C/EBP beta effectively blocked terminal differentiation. Finally, we have discovered that ectopic expression of C/EBP beta in multipotential NIH-3T3 cells results in their conversion into committed adipoblasts capable, upon hormonal stimulation, of synchronous and uniform differentiation into fat-laden adipocytes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/06/20 alle ore 01:33:47