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Titolo:
DETERMINATION OF FLUMAZENIL IN PLASMA BY GAS CHROMATOGRAPHY-NEGATIVE ION CHEMICAL-IONIZATION MASS-SPECTROMETRY
Autore:
SONG DM; KHAYKIS V; KOHLHOF K;
Indirizzi:
CLIN RES ASSOCIATES,50 MADISON AVE NEW YORK NY 10010
Titolo Testata:
Journal of chromatography B. Biomedical applications
fascicolo: 2, volume: 663, anno: 1995,
pagine: 263 - 273
Fonte:
ISI
Lingua:
ENG
Soggetto:
BENZODIAZEPINE ANTAGONIST; MIDAZOLAM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
8
Recensione:
Indirizzi per estratti:
Citazione:
D.M. Song et al., "DETERMINATION OF FLUMAZENIL IN PLASMA BY GAS CHROMATOGRAPHY-NEGATIVE ION CHEMICAL-IONIZATION MASS-SPECTROMETRY", Journal of chromatography B. Biomedical applications, 663(2), 1995, pp. 263-273

Abstract

A gas chromatographic-negative ion chemical ionization mass spectrometric (GC-NCI-MS) method for the determination of flumazenil in plasma is described. The GC of flumazenil (M(r) 303) is considered to be difficult as it is readily adsorbed in the GC column. Therefore, preconditioning the GC column with reconstituted extract from plasma and Silyl-8 was required to cover the active sites on the column. Monitoring themaximum mass peak (m/z 275) of the flumazenil resulted in a tenfold enhancement of sensitivity and signal-to-noise ratio (concentration = 1ng/ml). Isotopically labeled flumazenil-d(3) (M(r) 306, m/z 278) was used as the internal standard. The detection limit for flumazenil was found to be 0.1 ng/ml with an injection volume of 2 mu l. The signal-to-noise ratio was about 10. The routine quantification limit was set at 2 ng/ml for dog plasma and 1 ng/ml for human plasma. The sample volumes in both instances were 1 ml.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 12:50:50