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Titolo:
BIFUNCTIONAL AND MULTIMERIC COMPLEXES OF STREPTAVIDIN FUSED TO SINGLE-CHAIN ANTIBODIES (SCFV)
Autore:
DUBEL S; BREITLING F; KONTERMANN R; SCHMIDT T; SKERRA A; LITTLE M;
Indirizzi:
GERMAN CANC RES CTR,RECOMBINANT ANTIBODY RES UNIT,NEUENHEIMER FELD 506 HEIDELBERG GERMANY GERMAN CANC RES CTR,RECOMBINANT ANTIBODY RES UNIT HEIDELBERG GERMANY UNIV HEIDELBERG,INST MOLEC GENET D-69120 HEIDELBERG GERMANY MAX PLANCK INST BIOPHYS D-60528 FRANKFURT GERMANY
Titolo Testata:
Journal of immunological methods
fascicolo: 2, volume: 178, anno: 1995,
pagine: 201 - 209
SICI:
0022-1759(1995)178:2<201:BAMCOS>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI; SURFACE; FV; CONSTRUCTION; EXPRESSION;
Keywords:
SINGLE CHAIN FV ANTIBODY; EXPRESSION IN ESCHERICHIA COLI; CHROMATOGRAPHY, IMMOBILIZED METAL AFFINITY; STREPTAVIDIN; IMMUNOCONJUGATE; BIOTIN; BIFUNCTIONAL ANTIBODY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
22
Recensione:
Indirizzi per estratti:
Citazione:
S. Dubel et al., "BIFUNCTIONAL AND MULTIMERIC COMPLEXES OF STREPTAVIDIN FUSED TO SINGLE-CHAIN ANTIBODIES (SCFV)", Journal of immunological methods, 178(2), 1995, pp. 201-209

Abstract

Multivalent and multispecific antibodies with defined stoichiometry could provide valuable tools for biological and medical research and for the diagnosis and therapy of cancer. We have therefore fused single chain antibodies (scFv) with core-streptavidin. This chimeric protein,expressed by the vector pSTE-215 (plasmid for streptavidin tagged expression), can form tetrameric complexes, binds antigen and contains the biotin binding site which may be used for further complex formation. An additional cysteine was inserted near the carboxy terminus to facilitate the construction of covalently linked bifunctional molecules. The scFv fusion protein could be purified by affinity chromatography using biotin analogues. We have also shown that the scFv fusion protein could be used for direct detection of its antigen in ELISA and Westernblots when stained with biotinylated horseradish peroxidase.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/05/20 alle ore 12:47:11