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Titolo:
EXCITOCHEMICAL-INDUCED TROPHIC BRIDGING DIRECTS AXONAL GROWTH OF TRANSPLANTED NEURONS TO DISTAL TARGET
Autore:
ZHOU FC; CHIANG YH;
Indirizzi:
INDIANA UNIV,SCH MED,DEPT ANAT,635 BARNHILL DR INDIANAPOLIS IN 46202 INDIANA UNIV,SCH MED,PROGRAM MED NEUROBIOL INDIANAPOLIS IN 46202
Titolo Testata:
Cell transplantation
fascicolo: 1, volume: 4, anno: 1995,
pagine: 103 - 112
SICI:
0963-6897(1995)4:1<103:ETBDAG>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADULT-RAT BRAIN; STRIATAL GRAFTS; NEURAL TRANSPLANTATION; CONNECTIVITIES; ASTROCYTES; RECOVERY; LAMININ;
Keywords:
BRAIN GRAFT; NEURAL BRIDGING; AXONAL GUIDANCE; NEUROTROPHIC FACTOR; KAINIC ACID; IBOTENIC ACID; PARKINSONISM; STRIATUM; SUBSTANTIA NIGRA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
F.C. Zhou e Y.H. Chiang, "EXCITOCHEMICAL-INDUCED TROPHIC BRIDGING DIRECTS AXONAL GROWTH OF TRANSPLANTED NEURONS TO DISTAL TARGET", Cell transplantation, 4(1), 1995, pp. 103-112

Abstract

Brain grafts directly placed in their target regions often lack proper connections and normal regulation. When brain grafts are placed in their ontogenetic normal area, the axonal outgrowth of grafts to distaltarget regions is a major obstacle. We previously demonstrated that directional axonal growth of grafts can be facilitated by laminin. In this study we demonstrated that excitochemicals induced trophic environment can be strategically used as a bridge to guide transplanted neurons to innervate a distal target of millimeter distance, and has a unique effect on fiber expansion in the target region. To distinguish dopamine (DA) growth from that of transplants, 6-hdroxydopamine was unilaterally injected into medial forebrain bundle to remove dopamine innervation to the striatum. Ibotenic acid (IB), kainic acid (KA), or phosphate buffer (vehicle) were microinjected through a glass-pipet to make a 7 mm long tract between the substantia nigra and the striatum in Sprague-Dawley rats. Gestational 14-15 days brainstem slurry was transplanted either in the same or a separate tract into nigra at the bottom of the bridge. All transplants survived with abundant serotonin (5-HT) and DA neurons. Traceable DA fibers formed distinct bundles from grafts along the length of IB/KA tracts. Upon arrival at their major targetregions, fibers from bundles were capable of leaving the tracts, and reinnervating the DA-vacated striatum. The 5-HT fibers formed bundles similar to DA fibers but left the tract earlier upon arrival at their major target the globus pallidus and when arriving at the striatum andcortex. Fibers from grafts with vehicle tracts grew randomly and not into the tract. These results indicate that the trophic effects of chemical lesioning serves as a remarkably effective axonal guidance for more than one type of fetal neuron to innervate distal brain regions. This method has great potential in directing fibers in many transplant models.

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Documento generato il 02/04/20 alle ore 02:33:25