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Titolo:
REGULATION OF PROLIFERATION AND PRODUCTION OF PROSTATE-SPECIFIC ANTIGEN IN ANDROGEN-SENSITIVE PROSTATIC-CANCER CELLS, LNCAP, BY DIHYDROTESTOSTERONE
Autore:
LEE C; SUTKOWSKI DM; SENSIBAR JA; ZELNER D; KIM I; AMSEL I; SHAW N; PRINS GS; KOZLOWSKI JM;
Indirizzi:
NORTHWESTERN UNIV,SCH MED,DEPT UROL,TARRY 11-715,303 E CHICAGO AVE CHICAGO IL 60611 ABBOTT LABS,DIV CANC DIAGNOST ABBOTT PK IL 60064 UNIV ILLINOIS,MED CTR,DEPT OBSTET & GYNECOL CHICAGO IL 60616
Titolo Testata:
Endocrinology
fascicolo: 2, volume: 136, anno: 1995,
pagine: 796 - 803
SICI:
0013-7227(1995)136:2<796:ROPAPO>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPIDERMAL GROWTH-FACTOR; EPITHELIAL-CELLS; RAT PROSTATE; CATHEPSIN-D; PROTEASE INHIBITORS; RECEPTOR; GENE; LINE; EXPRESSION; PROLACTIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
C. Lee et al., "REGULATION OF PROLIFERATION AND PRODUCTION OF PROSTATE-SPECIFIC ANTIGEN IN ANDROGEN-SENSITIVE PROSTATIC-CANCER CELLS, LNCAP, BY DIHYDROTESTOSTERONE", Endocrinology, 136(2), 1995, pp. 796-803

Abstract

LNCaP is an androgen-sensitive human prostatic cancer cell line. The effect of androgen on these cells is characterized by a bell-shaped growth response and a dose-dependent induction of prostate-specific antigen (PSA) production. The present study was carried out to gain further insight into the effect of androgen on LNCaP. Cells were cultured inphenol red-free RPMI-1640 supplemented with 10% charcoal-stripped fetal bovine serum, with concentrations of dihydrotestosterone (DHT) ranging from 0-10(-7) M, in a 4-day culture system. A bell-shaped growth response was reproduced with a peak level of cell count at 10(-10) M DHT. PSA secretion from these cells did not increase significantly untilthe DHT level in the medium reached 10(-9) M. A progressive increase in PSA secretion was observed at higher DHT concentrations accompaniedwith a progressive decline in cellular proliferation. The results of immunocytochemical analysis of PSA localization indicated that the proportion of cells with positive staining for PSA also increased with increasing concentrations of DHT. Analysis of androgen receptors, as determined by both immunocytochemistry and Western blot analysis, showed a decline in nuclear androgen receptor at low concentrations of DHT and an increase in the amount of receptor protein at high concentrations. These results indicated that the androgen-induced bell-shaped growthresponse in LNCaP cells represented the manifestation of two different cellular events in dose-related manner: cellular proliferation at low DHT concentrations and increased production of PSA at high DHT concentrations.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/04/20 alle ore 12:18:15