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Titolo:
PREPARATION AND CHARACTERIZATION OF A DEOXYOLIGONUCLEOTIDE 49-MER CONTAINING A SITE-SPECIFIC THYMIDYLYL-(3',5')-DEOXYADENOSINE PHOTOPRODUCT
Autore:
ZHAO XD; KAO JLF; TAYLOR JS;
Indirizzi:
WASHINGTON UNIV,DEPT CHEM ST LOUIS MO 63130 WASHINGTON UNIV,DEPT CHEM ST LOUIS MO 63130
Titolo Testata:
Biochemistry
fascicolo: 4, volume: 34, anno: 1995,
pagine: 1386 - 1392
SICI:
0006-2960(1995)34:4<1386:PACOAD>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
SYN THYMINE DIMER; CIS-SYN; ULTRAVIOLET-LIGHT; DNA; SPECTROSCOPY; SEQUENCES; NMR; PHOTOREACTIVITY; PHOTOCHEMISTRY; ELUCIDATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
X.D. Zhao et al., "PREPARATION AND CHARACTERIZATION OF A DEOXYOLIGONUCLEOTIDE 49-MER CONTAINING A SITE-SPECIFIC THYMIDYLYL-(3',5')-DEOXYADENOSINE PHOTOPRODUCT", Biochemistry, 34(4), 1995, pp. 1386-1392

Abstract

Irradiation of d(GTATTATG) with 254 nm light gave rise to four major photoproducts, two of which were readily identified by NMR as the cia-syn cyclobutane dimer and the (6-4) photoproduct of the central TT site, Analysis of the NMR data for the other two photoproducts indicated that they were not any of the other known photoproducts of a TT site and might be TA photoproducts [Bose, S, N., et al. (1983) Science 220,723-725]. In support of this possibility, the fluorescence spectra ofthe products of acid hydrolysis of the two photoproducts were very similar to that reported for the hydrolysis product of the TA photoproduct of TpdA. Only one of the two TA-containing octamers could be ligated at both ends to form a 49-mer oligonucleotide in the presence of acomplementary oligonucleotide scaffold, suggesting that the TA photoproduct had formed between T5 and A6. The position of the TA photoproduct was confirmed by mapping the arrest sites for 3'-->5' exonucleolytic degradation of the 49-mer by T4 DNA polymerase and for primer extension opposite the 49-mer by exonuclease deficient Klenow fragment (KF) and Sequenase Version 2.0., The TA product could also be bypassed by both polymerases, but it was less of a block to KF. Treatment with 1M aqueous piperidine at 100 degrees C led to a maximum of about 34% cleavage of the DNA at the site of the TA product. The H-1 NMR signalsof d(GTATTATG) were assigned by TOCSY, NOESY, and H-1-P-31 COSY, andthe H-1 NMR shifts for the TA photoproduct were comparable to those reported for the dinucleotide photoproduct [Koning, T. hi. G., et al. (1990) Nucleic Acids Res. 18, 277-284]. The biological implications ofthis photoproduct are also discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 06:17:33