Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
GENETIC APPROACH TO ENHANCED BIOLOGICAL PHOSPHORUS REMOVAL
Autore:
OHTAKE H; YAMADA K; HARDOYO; MURAMATSU A; ANBE Y; KATO J; SHINJO H;
Indirizzi:
HIROSHIMA UNIV,DEPT FERMENTAT TECHNOL HIGASHIHIROSHIMA 724 JAPAN EBARA RES CO LTD,ENVIRONM PROTECT RES INST FUJISAWA KANAGAWA 251 JAPAN
Titolo Testata:
Water science and technology
fascicolo: 6, volume: 30, anno: 1994,
pagine: 185 - 192
SICI:
0273-1223(1994)30:6<185:GATEBP>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI; POLYPHOSPHATE KINASE; ACINETOBACTER; PHOSPHATE;
Keywords:
BACTERIA; BIOLOGICAL PHOSPHORUS REMOVAL; EXOPOLYPHOSPHATASE; GENE MANIPULATION; PHOSPHATE TRANSPORT SYSTEM; POLYPHOSPHATE KINASE; POLYPHOSPHATE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
12
Recensione:
Indirizzi per estratti:
Citazione:
H. Ohtake et al., "GENETIC APPROACH TO ENHANCED BIOLOGICAL PHOSPHORUS REMOVAL", Water science and technology, 30(6), 1994, pp. 185-192

Abstract

Genetic improvement of bacterial ability to accumulate phosphate (Pi)was investigated using Escherichia coli as a test organism. High levels of Pi accumulation were achieved by (i) modifying the genetic regulation and increasing the dosage of the E. coli genes encoding polyphosphate kinase (ppk), acetate kinase (ackA), and the phosphate inducibletransport system (pstS, pstC, pstA, and pstB) and (ii) genetically inactivating ppx encoding exopolyphosphatase. Acetate kinase was employed as an ATP regeneration system for polyphosphate synthesis. The best recombinant strain, which contained both pBC29 (ppk) and pEP02.2 (pst genes) accumulated approximately 10-fold more Pi than did the control strain. The phosphorus content of this recombinant reached a maximum of 16 % on the dry weight basis (49 % as phosphate). About 65 % of the cellular phosphorus was stored as polyphosphate.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 12:32:12