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Titolo:
IDENTIFICATION OF A MOLECULE OF PORPHYROMONAS-GINGIVALIS THAT BINDS TO STREPTOCOCCUS-GORDONII
Autore:
LAMONT RJ; HSIAO GW; GIL S;
Indirizzi:
UNIV WASHINGTON,SCH DENT,DEPT ORAL BIOL SEATTLE WA 98195
Titolo Testata:
Microbial pathogenesis
fascicolo: 5, volume: 17, anno: 1994,
pagine: 355 - 360
SICI:
0882-4010(1994)17:5<355:IOAMOP>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
BACTEROIDES-GINGIVALIS; ORAL STREPTOCOCCI; ESCHERICHIA-COLI; ADHERENCE; FIMBRIAE; PROTEINS; ASACCHAROLYTICUS; PURIFICATION; ANTIGENS; BIOTIN;
Keywords:
PORPHYROMONAS GINGIVALIS; STREPTOCOCCUS GORDONII; ADHESIN;
Tipo documento:
Note
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
17
Recensione:
Indirizzi per estratti:
Citazione:
R.J. Lamont et al., "IDENTIFICATION OF A MOLECULE OF PORPHYROMONAS-GINGIVALIS THAT BINDS TO STREPTOCOCCUS-GORDONII", Microbial pathogenesis, 17(5), 1994, pp. 355-360

Abstract

The molecules that mediate the adherence of Porphyromonas gingivalis,a periodontal pathogen, to Streptococcus gordonii, a commensal plaqueorganism, were investigated. Outer membrane proteins of P. gingivaliswere labelled with biotin, extracted by EDTA and reacted with S. gordonii cells. Interactive porphyromonas components were identified by SDS-PAGE of the S. gordonii cells followed by electroblotting and visualization of the adsorbed porphyromonas molecules with streptavidin-alkaline phosphatase. A P. gingivalis molecule of 35 kDa bound to S. gordonii. Monospecific polyclonal antibodies to the 35 kDa protein inhibited binding of P. gingivalis to S. gordonii by 71%. The antibodies also reacted with the P. gingivalis fimbriae, indicating that the 35 kDa molecule is antigenically related to, or associated with, the fimbriae.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 06:15:11