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Titolo:
COOPERATIVITY BETWEEN AN UPSTREAM TATA-LIKE SEQUENCE AND A CAA REPEATED ELEMENT MEDIATES E1A-DEPENDENT NEGATIVE REPRESSION OF THE H-2K(B) CLASS-I GENE
Autore:
TANG XR; LI HO; SAKATSUME O; OHTA T; TSUTSUI H; SMIT AFA; HORIKOSHI M; KOURILSKY P; ISRAEL A; GACHELIN G; YOKOYAMA K;
Indirizzi:
INST PHYS & CHEM RES,TSUKUBA LIFE SCI CTR,3-1-1 KOYADAI TSUKUBA IBARAKI 305 JAPAN INST PHYS & CHEM RES,TSUKUBA LIFE SCI CTR TSUKUBA IBARAKI 305 JAPAN CITY HOPE NATL MED CTR,BECKMAN RES INT,DEPT BIOL DUARTE CA 91010 UNIV TOKYO,INST MOLEC & CELLULAR BIOSCI,BUNKYO KU TOKYO 113 JAPAN INST PASTEUR,DEPT MOLEC BIOL,UNITE BIOL MOLEC EXPRESS GEN F-75724 PARIS 15 FRANCE INST PASTEUR,DEPT IMMUNOL,UNITE BIOL MOLEC GENE F-75724 PARIS 15 FRANCE
Titolo Testata:
The Journal of biological chemistry
fascicolo: 5, volume: 270, anno: 1995,
pagine: 2327 - 2336
SICI:
0021-9258(1995)270:5<2327:CBAUTS>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAJOR HISTOCOMPATIBILITY COMPLEX; NF-KAPPA-B; TRANSFORMED RAT-CELLS; DNA-BINDING PROTEIN; HEAT-SHOCK GENE; RETINOIC ACID; TRANSCRIPTIONAL ACTIVATION; ADENOVIRUS-TYPE-12 E1A; EXPRESSION; MHC;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
59
Recensione:
Indirizzi per estratti:
Citazione:
X.R. Tang et al., "COOPERATIVITY BETWEEN AN UPSTREAM TATA-LIKE SEQUENCE AND A CAA REPEATED ELEMENT MEDIATES E1A-DEPENDENT NEGATIVE REPRESSION OF THE H-2K(B) CLASS-I GENE", The Journal of biological chemistry, 270(5), 1995, pp. 2327-2336

Abstract

In primary rodent cells transformed by the E1A region of the highly oncogenic adenovirus type 12, repression of transcription mediated by the far upstream TATA-like element was observed only in conjunction with either possible juxtaposition of a CAA repeated element in the presence of E1A and was dependent upon the relative arrangement of both theTATA-like and CAA repeated motifs in both homologous and heterologouspromoter constructs, A gel shift competition study demonstrated that the TATA-binding protein (TBP) or a TBP-like protein can bind to both the upstream TATA-like sequence and the regular TATA box on the H-2K(b) basal promoter, Moreover, employing immunoselection and cyclic amplification and selection of targets (CASTing) methods with nuclear extracts derived from Ad12-E1A transformants, we have identified a high affinity binding site in the H-2K(b) class I promoter for E1A-associated DNA-binding proteins. The sequences of the binding sites were identified and were found to contain both the upstream TATA like motif and theCAA repeated motifs. Our results suggest that the TATA like sequence in the far upstream region of the H-2K(b) gene is one of the elements that is required for Ad12-E1A-mediated negative repression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 20:34:25