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Titolo:
NITROGEN REGULATION OF NASA AND THE NASB OPERON, WHICH ENCODE GENES REQUIRED FOR NITRATE ASSIMILATION IN BACILLUS-SUBTILIS
Autore:
NAKANO MM; YANG F; HARDIN P; ZUBER P;
Indirizzi:
LOUISIANA STATE UNIV,MED CTR,DEPT BIOCHEM & MOLEC BIOL,1501 KINGS HWYSHREVEPORT LA 71130
Titolo Testata:
Journal of bacteriology
fascicolo: 3, volume: 177, anno: 1995,
pagine: 573 - 579
SICI:
0021-9193(1995)177:3<573:NRONAT>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLUTAMINE-SYNTHETASE MUTANTS; CYANOBACTERIUM SYNECHOCOCCUS; NUCLEOTIDE-SEQUENCE; ANACYSTIS-NIDULANS; ALTERED REGULATION; L-ASPARAGINASE; L-ASPARTASE; ANS OPERON; GLNA GENE; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
M.M. Nakano et al., "NITROGEN REGULATION OF NASA AND THE NASB OPERON, WHICH ENCODE GENES REQUIRED FOR NITRATE ASSIMILATION IN BACILLUS-SUBTILIS", Journal of bacteriology, 177(3), 1995, pp. 573-579

Abstract

The divergently transcribed nasA gene and nasB operon are required for nitrate and nitrite assimilation in Bacillus subtilis. The beta-galactosidase activity of transcriptional lacZ fusions from the nasA and nasB promoters was high when cells were grown in minimal glucose mediumcontaining poor nitrogen sources such as nitrate, proline, or glutamate. The expression was very low when ammonium or glutamine was used asthe sole nitrogen source. The repression of the genes during growth on good sources of nitrogen required wild-type glutamine synthetase (GlnA), but not GlnR, the repressor of the glnRA operon. Primer extensionanalysis showed that the -10 region of each promoter resembles those of sigma(A)-recognized promoters. Between the divergently oriented nasA and nasB promoters is a region of dyad symmetry. Mutational analysisled to the conclusion that this sequence is required in cis for the activation of both nasA and nasB. The derepression of these genes in a glnA mutant also required this sequence. These results suggest that anunidentified transcriptional activator and glutamine synthetase function in the regulation of nasA and the nasB operon.

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Documento generato il 23/11/20 alle ore 21:34:06