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Titolo:
CCD MICROSCOPY AND IMAGE-ANALYSIS OF CELLS AND CHROMOSOMES STAINED BYFLUORESCENCE IN-SITU HYBRIDIZATION
Autore:
TANKE HJ; FLORIJN RJ; WIEGANT J; RAAP AK; VROLIJK J;
Indirizzi:
LEIDEN UNIV,DEPT CYTOCHEM & CYTOMETRY,WASSENAARSEWEG 72 2333 AL LEIDEN NETHERLANDS
Titolo Testata:
Histochemical Journal
fascicolo: 1, volume: 27, anno: 1995,
pagine: 4 - 14
SICI:
0018-2214(1995)27:1<4:CMAIOC>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
COMPARATIVE GENOMIC HYBRIDIZATION; MOLECULAR CYTOGENETIC ANALYSIS; INSITU HYBRIDIZATION; INTERPHASE NUCLEI; SOLID TUMORS; DNA PROBES; RESOLUTION; MULTIPLE; ABERRATIONS; RADIATION;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
H.J. Tanke et al., "CCD MICROSCOPY AND IMAGE-ANALYSIS OF CELLS AND CHROMOSOMES STAINED BYFLUORESCENCE IN-SITU HYBRIDIZATION", Histochemical Journal, 27(1), 1995, pp. 4-14

Abstract

This paper reviews methods and applications of CCD microscopy for analysing cells and chromosomes subjected to fluorescence in situ hybridization (FISH). The current status of indirect and direct FISH stainingmethods with respect to probe labelling, detection sensitivity, multiplicity and DNA resolution is summarized. Microscope hardware, including special multi-band pass filters and CCD cameras required for FISH analysis, is described. Then follows a detailed discussion of current and emerging applications such as the automated enumeration of chromosomal abnormalities (counting of dots in interphase cells), comparative genomic hybridization, automated evaluation of radiation-induced chromosomal translocations, and high-resolution DNA mapping on highly extended chromatin. Finally, the limitations of the present methodology andfuture prospects are discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 15:47:48