Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
RAPID ELECTROGENIC SULFATE-CHLORIDE EXCHANGE MEDIATED BY CHEMICALLY-MODIFIED BAND-3 IN HUMAN ERYTHROCYTES
Autore:
JENNINGS ML;
Indirizzi:
UNIV TEXAS,MED BRANCH,DEPT PHYSIOL & BIOPHYS GALVESTON TX 77555
Titolo Testata:
The Journal of general physiology
fascicolo: 1, volume: 105, anno: 1995,
pagine: 21 - 47
SICI:
0022-1295(1995)105:1<21:RESEMB>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
RED-BLOOD-CELL; ANION-EXCHANGE; MEMBRANE-POTENTIALS; TRANSPORT KINETICS; PROTEIN; SITE; COTRANSPORT; DEPENDENCE; MECHANISM; BINDING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
M.L. Jennings, "RAPID ELECTROGENIC SULFATE-CHLORIDE EXCHANGE MEDIATED BY CHEMICALLY-MODIFIED BAND-3 IN HUMAN ERYTHROCYTES", The Journal of general physiology, 105(1), 1995, pp. 21-47

Abstract

One of the modes of action of the red blood cell anion transport protein is the electrically silent net exchange of 1 Cl- for 1 SO4= and 1 H+. Net SO4=-Cl- exchange is accelerated by low pH or by conversion ofthe side chain of glutamate 681 into an alcohol by treatment of intact cells with Woodward's reagent K (WRK) and BH4-. The studies described here were performed to characterize the electrical propel ties of net SO4=-Cl- exchange in cells modified with WRK/BH4-. The SO4= conductance measured in 100 mM SO4= medium is smaller in modified cells than in control cells. However, the efflux of [S-35] SO4= into a 150-mM KCl medium is 80-fold larger in modified cells than in control cells and is inhibited 99% by 10 mu M H2DIDS. No detectable H+ flux is associatedwith SO4=-Cl- exchange in modified cells. In the presence of gramicidin to increase the cation permeability, the stoichiometry of SO4=-Cl- exchange is not distinguishable from 1:1. In modified cells loaded with SO4=, the valinomycin-mediated efflux of Rb-86(+) into an Nagluconate medium is immediately stimulated by the addition of 5 mM extracellular Cl-. Therefore, SO4=-Cl- exchange in modified cells causes an outward movement of negative charge, as expected for an obligatory 1:1 SO4=-Cl- exchange. This is the first example of an obligatory, electrogenic exchange process in band 3 and demonstrates that the coupling between influx and efflux does not require that the overall exchange be electrically neutral. The effects of membrane potential on SO4=-SO4= exchange and SO4=-Cl- exchange in modified cells are consistent with a model in which nearly a full net positive charge moves inward through the transmembrane field during the inward Cl- translocation event, and a small net negative charge moves with SO4= during the SO4= translocationevent. This result suggests that, in normal cells, the negative charge on Glu 681 traverses most of the transmembrane electric field, accompanied by Cl- and the equivalent of two protein-bound positive charges.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 17/01/21 alle ore 17:35:45