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Titolo:
FULL ACTIVE BACULOVIRUS-EXPRESSED HUMAN MONOCYTE CHEMOATTRACTANT PROTEIN-1 WITH THE INTACT N-TERMINUS
Autore:
ISHII K; YAMAGAMI S; TANAKA H; MOTOKI M; SUWA Y; ENDO N;
Indirizzi:
TEIJIN INST BIOMED RES,PHARMACEUT DISCOVERY RES LABS HINO TOKYO 191 JAPAN TEIJIN INST BIOMED RES,PHARMACEUT DISCOVERY RES LABS HINO TOKYO 191 JAPAN TEIJIN INST BIOMED RES,BIOTECHNOL RES LABS HINO TOKYO 191 JAPAN
Titolo Testata:
Biochemical and biophysical research communications
fascicolo: 3, volume: 206, anno: 1995,
pagine: 955 - 961
SICI:
0006-291X(1995)206:3<955:FABHMC>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
BLOOD MONONUCLEAR LEUKOCYTES; PURIFICATION; CLONING; VIRUS; CDNA; RNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
18
Recensione:
Indirizzi per estratti:
Citazione:
K. Ishii et al., "FULL ACTIVE BACULOVIRUS-EXPRESSED HUMAN MONOCYTE CHEMOATTRACTANT PROTEIN-1 WITH THE INTACT N-TERMINUS", Biochemical and biophysical research communications, 206(3), 1995, pp. 955-961

Abstract

The Sf-9 insect cells infected with a recombinant baculovirus integrated with a cDNA encoding human monocyte chemoattractant protein-1 (MCP-1) produced three different MCP-1s, which were isolated by reverse-phase HPLC as 13kDa, 14kDa, and 15kDa bands on SDS-PAGE. The heterogeneity between these MCP-1s was ascribed to the different degree of O-glycosylation with Gal beta 1-3GalNAc beta 1-Ser/Thr, while the 13kDa was regarded as carbohydrate-free. The integrity of the N-termius was confirmed by the amino acid sequence analysis. Each MCP-1 isolated showed equipotent monocyte chemotactic activity, which was rather higher thanthat of the E.coli-derived one. (C) 1995 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/10/20 alle ore 10:55:51