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Titolo:
LIPID-ACCUMULATION AND FOAM CELL-FORMATION IN CHINESE-HAMSTER OVARY CELLS OVEREXPRESSING VERY-LOW-DENSITY LIPOPROTEIN RECEPTOR
Autore:
SUZUKI J; TAKAHASHI S; OIDA K; SHIMADA A; KOHNO M; TAMAI T; MIYABO S; YAMAMOTO T; NAKAI T;
Indirizzi:
FUKUI MED SCH,DEPT INTERNAL MED 3 MATSUOKA FUKUI 91011 JAPAN FUKUI MED SCH,DEPT INTERNAL MED 3 MATSUOKA FUKUI 91011 JAPAN TOHOKU UNIV,CTR GENE RES SENDAI MIYAGI 981 JAPAN
Titolo Testata:
Biochemical and biophysical research communications
fascicolo: 3, volume: 206, anno: 1995,
pagine: 835 - 842
SICI:
0006-291X(1995)206:3<835:LAFCIC>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOUSE PERITONEAL-MACROPHAGES; HUMAN MONOCYTE-MACROPHAGES; MEDIATED ENDOCYTOSIS; REMNANT LIPOPROTEINS; CHOLESTERYL ESTER; CANINE BETA; DEGRADATION; PATHWAY; PROTEIN; RICH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
J. Suzuki et al., "LIPID-ACCUMULATION AND FOAM CELL-FORMATION IN CHINESE-HAMSTER OVARY CELLS OVEREXPRESSING VERY-LOW-DENSITY LIPOPROTEIN RECEPTOR", Biochemical and biophysical research communications, 206(3), 1995, pp. 835-842

Abstract

The rabbit very low density lipoprotein receptor gene was introduced into LDL receptor-negative Chinese hamster ovary cells (ldl-A7). Incubation of the transfected cells with rabbit B-VLDL (5 to 8 mu g protein/ml), for 6 days, induced foam cell formation. The cells accumulated lipid droplets visualized by oil red-O staining; the cellular cholesteryl ester and triglyceride content increased two- to threefold. [H-3]Oleate incorporation into cholesteryl [H-3]oleate was stimulated threefold by incubation of cells with beta-VLDL (20 mu g protein/ml). Northern blot analysis revealed the presence of VLDL receptor mRNA in rabbit resident alveolar macrophages. Incubation of the macrophages with beta-VLDL (20 mu g protein/ml) for 24 hours induced foam cell formation but had virtually no effect on VLDL receptor mRNA abundance. These results suggest that the VLDL receptor on macrophages may play an importantrole in foam cell formation. (C) 1995 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/02/20 alle ore 11:34:43