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Titolo:
CHINESE-HAMSTER OVARY CELLS EXPRESSING A NOVEL TYPE OF ACETYLATED LOW-DENSITY-LIPOPROTEIN RECEPTOR - ISOLATION AND CHARACTERIZATION
Autore:
FUKASAWA M; HIROTA K; ADACHI H; MIMURA K; MURAKAMIMUROFUSHI K; TSUJIMOTO M; ARAI H; INOUE K;
Indirizzi:
UNIV TOKYO,FAC PHARMACEUT SCI,DEPT HLTH CHEM,BUNKYO KU,7-3-1 HONGO TOKYO 113 JAPAN UNIV TOKYO,FAC PHARMACEUT SCI,DEPT HLTH CHEM,BUNKYO KU TOKYO 113 JAPAN SUNTORY INST BIOMED RES MISHIMA 618 SHIZUOKA JAPAN OCHANOMIZU UNIV,FAC SCI,DEPT BIOL,BUNKYO KU TOKYO 112 JAPAN
Titolo Testata:
The Journal of biological chemistry
fascicolo: 4, volume: 270, anno: 1995,
pagine: 1921 - 1927
SICI:
0021-9258(1995)270:4<1921:COCEAN>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
MACROPHAGE SCAVENGER RECEPTORS; HUMAN MONOCYTE-MACROPHAGES; HOMOZYGOUS FAMILIAL HYPERCHOLESTEROLEMIA; SMOOTH-MUSCLE CELLS; ENDOTHELIAL-CELLS; MEDIATED ENDOCYTOSIS; BINDING-PROPERTIES; LIGAND-BINDING; CULTURED-CELLS; DEGRADATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
M. Fukasawa et al., "CHINESE-HAMSTER OVARY CELLS EXPRESSING A NOVEL TYPE OF ACETYLATED LOW-DENSITY-LIPOPROTEIN RECEPTOR - ISOLATION AND CHARACTERIZATION", The Journal of biological chemistry, 270(4), 1995, pp. 1921-1927

Abstract

Macrophage scavenger receptors mediate the recognition of a wide range of negatively charged macromolecules including acetylated low density lipoproteins (AcLDL). Chinese hamster ovary (CHO) cells were cultured in the presence of increasing concentrations of simvastatin, a cholesterol biosynthesis inhibitor, and AcLDL as the sole source of exogenous Lipoproteins. The cells surviving under these conditions specifically bound I-125-labeled AcLDL with high affinity and degraded them via an endocytic pathway, Unexpectedly, the association and degradation ofI-125-labeled AcLDL by these CHO cells were not inhibited by dextran sulfate, fucoidan, and polyinosinic acid, competitors of macrophage scavenger receptors, but were completely inhibited by maleylated bovine serum albumin. Furthermore, these cells effectively took up negativelycharged liposomes containing acidic phospholipids such as phosphatidylserine and phosphatidic acid, whereas CHO cells expressing macrophagescavenger receptors did not. AcLDL and negatively charged liposomes were cross-competed with each other. Northern blot analysis using the cDNA for the macrophage scavenger receptor revealed that these CHO cells did not express this receptor. From these observations, we conclude that the isolated CHO cells express a novel type of AcLDL receptor, which is distinct from macrophage scavenger receptors with respect to ligand specificity and competitor sensitivity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/02/20 alle ore 14:16:03