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Titolo:
THE ROLE OF CARDIAC MITOCHONDRIA IN THE REGULATION OF INTRACELLULAR CALCIUM DURING ISCHEMIA AND REPERFUSION - X-RAY-MICROANALYSIS USING FREEZE-DRIED SECTIONS
Autore:
HORIKAWA Y; KANEKO N; HOSODA S;
Indirizzi:
TOKYO WOMENS MED COLL,DEPT CARDIOL,8-1 KAWADA CHO,SHINJUKU KU TOKYO 162 JAPAN
Titolo Testata:
Heart and vessels
fascicolo: 1, volume: 10, anno: 1995,
pagine: 1 - 6
SICI:
0910-8327(1995)10:1<1:TROCMI>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Keywords:
CALCIUM; MITOCHONDRIA; ISCHEMIA; REPERFUSION; X-RAY MICROANALYSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
NO
Recensione:
Indirizzi per estratti:
Citazione:
Y. Horikawa et al., "THE ROLE OF CARDIAC MITOCHONDRIA IN THE REGULATION OF INTRACELLULAR CALCIUM DURING ISCHEMIA AND REPERFUSION - X-RAY-MICROANALYSIS USING FREEZE-DRIED SECTIONS", Heart and vessels, 10(1), 1995, pp. 1-6

Abstract

The calcium concentration in papillary muscles was measured by X-ray microanalysis in order to clarify the role played by mitochondria in intracellular calcium regulation during ischemia and reperfusion. Rat hearts perfused by the Langendorff method were rapidly frozen prior to and during ischemia, as well as following reperfusion. Sections prepared by cryoultramicrotomy were freeze-dried, carbon-coated, and analyzed in an electron microscope. A new freeze-drying procedure was developed, in which the ultrastructure was well-preserved, with sarcomeres, triads, and mitochondria easily recognized. Calcium accumulation into the mitochondria occurred during 30-min ischemia (29.7 +/- 17.0 mmol/kgdry weight) and increased further after 15-min reperfusion (157.1 +/-104.5), the calcium concentration decreased after 60-min reperfusion (58.1 +/- 29.0). However, the calcium concentration in the cytosol didnot change significantly. It is thought that mitochondrial calcium accumulation is reversible, to a certain degree, and that the mitochondria play a part in intracellular calcium regulation in pathological states.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 20:46:04