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Titolo:
CHARACTERIZATION OF THE ASSOCIATION OF PHOSPHOLIPASE C-DELTA WITH ALZHEIMER NEUROFIBRILLARY TANGLES
Autore:
SHIMOHAMA S; PERRY G; RICHEY P; PRAPROTNIK D; TAKENAWA T; FUKAMI K; WHITEHOUSE PJ; KIMURA J;
Indirizzi:
KYOTO UNIV,FAC MED,DEPT NEUROL,SAKYO KU,54 SHOGOINKAWAHARACHO KYOTO 606 JAPAN CASE WESTERN RESERVE UNIV,UNIV HOSP CLEVELAND,INST PATHOL,DIV NEUROPATHOL CLEVELAND OH 44106 UNIV TOKYO,INST MED,DEPT ONCOBIOL,MINATO KU TOKYO 108 JAPAN CASE WESTERN RESERVE UNIV,UNIV HOSP CLEVELAND,DEPT NEUROL CLEVELAND OH 44106
Titolo Testata:
Brain research
fascicolo: 2, volume: 669, anno: 1995,
pagine: 217 - 224
SICI:
0006-8993(1995)669:2<217:COTAOP>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
PAIRED HELICAL FILAMENTS; CELL ANTIGEN RECEPTOR; SIGNAL TRANSDUCTION; TAU-PROTEIN; ZETA-CHAIN; DISEASE; UBIQUITIN; EPITOPES; SENILE; ACCUMULATION;
Keywords:
ALZHEIMERS DISEASE; PHOSPHOLIPASE C-DELTA; TAU; BASIC FIBROBLAST GROWTH FACTOR; ELECTRON MICROSCOPY; SOLUBILIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
S. Shimohama et al., "CHARACTERIZATION OF THE ASSOCIATION OF PHOSPHOLIPASE C-DELTA WITH ALZHEIMER NEUROFIBRILLARY TANGLES", Brain research, 669(2), 1995, pp. 217-224

Abstract

Phosphoinositide-specific phospholipase C (PLC) is a key enzyme in signal transduction. We have previously demonstrated that an antibody tothe PLC isozyme, PLC-delta, intensely stained neurofibrillary tangles(NFT) in the brain tissue of AD patients [Am. J. Pathol, 139 (1991) 737-742]. To clarify the crucial involvement of abnormal PLC-delta accumulation contributing to the formation of NFT, we performed light and electron microscopic immunocytochemistry. To determine PLC-delta's association with NFT, its resistance to solubilization was also studied. Anti-PLC-delta antibody marked the same NFT-bearing neurons containingtau immunoreactivity with tau more clearly on NFT filaments and PLC-delta covering it superficially at the light microscope level. The double stained preparations with anti-PLC-delta antibody and bFGF binding suggested that PLC-delta is an intracellular marker and is not retained after neuronal death. Employing immunoperoxidase and immunogold electron microscopic immunocytochemistry, we found that the antibody to PLC-delta reacts mostly with amorphous granular materials, and occasionally with some abnormal filaments within NFT. Nevertheless, PLC-delta in NFT was resistant to removal by high salt or ionic detergent, indicating it is an integral NFT component. These results indicate that antigenic determinants unique to PLC-delta are mainly present intraneuronally on the amorphous granular components of NFT as well as the abnormal filaments, suggesting PLC-delta's interactions and possible role in the formation of intraneuronal filamentous inclusions in AD.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 23:58:52