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Titolo:
CHANGES IN GENE-EXPRESSION IN RESPONSE TO SUCROSE ACCUMULATION IN LEAF TISSUE OF LOLIUM-TEMULENTUM L
Autore:
WINTERS AL; WILLIAMS JHH; THOMAS DS; POLLOCK CJ;
Indirizzi:
AFRC,INST GRASSLAND & ENVIRONM RES,PLAS GOGERDDAN ABERYSTWYTH SY23 3EB DYFED WALES
Titolo Testata:
New phytologist
fascicolo: 4, volume: 128, anno: 1994,
pagine: 591 - 600
SICI:
0028-646X(1994)128:4<591:CIGIRT>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
FRUCTAN BIOSYNTHESIS; EXCISED LEAVES; METABOLISM; PROTEINS; INDUCTION; CEREALS; BARLEY;
Keywords:
SUCROSE; FRUCTAN; GENE EXPRESSION; SOURCE LEAVES; TRANSCRIPTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
A.L. Winters et al., "CHANGES IN GENE-EXPRESSION IN RESPONSE TO SUCROSE ACCUMULATION IN LEAF TISSUE OF LOLIUM-TEMULENTUM L", New phytologist, 128(4), 1994, pp. 591-600

Abstract

Sucrose accumulation and subsequent fructan synthesis was initiated in detached, mature leaves of Lolium temulentum by incubating them either in the light or with 200 mM sucrose in the dark. Fructan synthesis was also induced in intact seedlings by lowering root and shoot meristem temperature to 5 degrees C while maintaining aerial parts of the plant at 20 degrees C in the light. In this system, subsequent breakdownof fructans was induced by raising root and meristem temperature to 20 OC in the dark. Products of in vitro translations showed that transcripts encoding polypeptides of 17 and 35 kDa increased in sucrose accumulating-tissue. Transcripts encoding 28 and 32 kDa polypeptide products appeared to be associated with leaf tissue actively exporting carbohydrate. Labelling studies in vivo in mature leaf tissue showed transient increases in the synthesis of polypeptides with MR 59, 60 and 70 kDa during the first 6 h of sucrose accumulation. Polypeptides with an MR of 74 and 27 kDa showed increased amounts later in induction. Cordycepin and cycloheximide were fed to mature leaf tissue at different stages during induction of sucrose accumulation in the light. Both control and cordycepin-treated tissue showed higher levels of monosaccharide accumulation compared with cycloheximide-treated tissue. Cordycepin applied during the first 2 h after excision blocked subsequent synthesis of all fructans. When applied at two-hour-stages between 2 and 8 h,subsequent accumulation of 1-kestose occurred without accumulation ofhigher fructans. Application after 8 h had no effect upon the patternof fructan accumulation when compared to untreated controls. Application of cycloheximide during the first 8 h following excision blocked all fructan accumulation. Subsequent administration had no effect on the pattern of fructan accumulation. The results are discussed in relation to the changes in gene expression associated with induction of fructan synthesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 12:36:29