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Titolo:
INTERFERON REGULATORY FACTOR-1 IS REQUIRED FOR MOUSE GBP GENE ACTIVATION BY GAMMA-INTERFERON
Autore:
BRIKEN V; RUFFNER H; SCHULTZ U; SCHWARZ A; REIS LFL; STREHLOW I; DECKER T; STAEHELI P;
Indirizzi:
UNIV FREIBURG,VIROL ABT,INST MIKROBIOL & HYG,HERMANN HERDER STR 11 D-79008 FREIBURG GERMANY UNIV FREIBURG,VIROL ABT,INST MIKROBIOL & HYG D-79008 FREIBURG GERMANY UNIV ZURICH,INST MOLEK BIOL 1 CH-8092 HONGG SWITZERLAND BIOZENTRUM WIEN,INST MIKROBIOL A-1020 VIENNA AUSTRIA
Titolo Testata:
Molecular and cellular biology
fascicolo: 2, volume: 15, anno: 1995,
pagine: 975 - 982
SICI:
0270-7306(1995)15:2<975:IRFIRF>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
GUANYLATE-BINDING PROTEIN; IFN-INDUCIBLE GENES; TRANSCRIPTION FACTOR; MOLECULAR-CLONING; MESSENGER-RNAS; BETA GENES; FACTOR-I; IRF-1; INDUCTION; ALPHA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
58
Recensione:
Indirizzi per estratti:
Citazione:
V. Briken et al., "INTERFERON REGULATORY FACTOR-1 IS REQUIRED FOR MOUSE GBP GENE ACTIVATION BY GAMMA-INTERFERON", Molecular and cellular biology, 15(2), 1995, pp. 975-982

Abstract

Full-scale transcriptional activation of the mouse Gbp genes by gammainterferon (IFN-gamma) requires protein synthesis in embryonic fibroblasts. Although the Gbp-1 and Gbp-2 promoters contain binding sites for transcription factors Stat1 and IFN regulatory factor 1 (IRF-1), deletion analysis revealed that the Stat1 binding site is dispensable forIFN-gamma inducibility of Gbp promoter constructs in transfected fibroblasts. However, activation of the mouse Gbp promoter by IFN-gamma requires transcription factor IRF-1. Transient overexpression of IRF-1 cDNA in mouse fibroblasts resulted in high-level expression of Gbp promoter constructs. Unlike wild-type cells, IRF-1(0/0) embryonic stem cells lacking functional transcription factor IRF-1 contained very low levels of Gbp transcripts that were not increased in response to differentiation or treatment with IFN-gamma. Treatment of IRF-1(0/0) mice with IFN-gamma resulted in barely detectable levels of Gbp RNA in spleens, lungs, and livers, whereas such treatment induced high levels of GbpRNA in the organs of wild-type mice. These observations suggest two alternative pathways for transcriptional induction of genes in responseto IFN-gamma: immediate response that results from activation of preformed Stat1 and delayed response that results from induced de novo synthesis of transcription factor IRF-1.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/01/20 alle ore 14:40:58