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Titolo:
THE ERYTHROID KRUPPEL-LIKE FACTOR TRANSACTIVATION DOMAIN IS A CRITICAL COMPONENT FOR CELL-SPECIFIC INDUCIBILITY OF A BETA-GLOBIN PROMOTER
Autore:
BIEKER JJ; SOUTHWOOD CM;
Indirizzi:
CUNY MT SINAI SCH MED,BROOKDALE CTR MOLEC BIOL,BOX 1126,1 GUSTAVE L LEVY PL NEW YORK NY 10029 CUNY MT SINAI SCH MED,DEPT BIOCHEM NEW YORK NY 10029
Titolo Testata:
Molecular and cellular biology
fascicolo: 2, volume: 15, anno: 1995,
pagine: 852 - 860
SICI:
0270-7306(1995)15:2<852:TEKFTD>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
MURINE ERYTHROLEUKEMIA-CELLS; EUKARYOTIC TRANSCRIPTIONAL ACTIVATORS; REGULATORY ELEMENT UPSTREAM; DOMINANT CONTROL REGION; LOCUS-CONTROL REGION; HUMAN GAMMA-GLOBIN; TRANSGENIC MICE; DNA-BINDING; DEVELOPMENTAL REGULATION; NUCLEAR PROTEINS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
69
Recensione:
Indirizzi per estratti:
Citazione:
J.J. Bieker e C.M. Southwood, "THE ERYTHROID KRUPPEL-LIKE FACTOR TRANSACTIVATION DOMAIN IS A CRITICAL COMPONENT FOR CELL-SPECIFIC INDUCIBILITY OF A BETA-GLOBIN PROMOTER", Molecular and cellular biology, 15(2), 1995, pp. 852-860

Abstract

Erythroid Kruppel-like factor (EKLF) is an erythroid cell-specific DNA-binding protein that activates transcription from the beta-globin CACCC element, a functionally important and evolutionarily conserved component of globin as well as other erythroid cell-specific promoters and enhancers, We have attempted to elucidate the molecular role of EKLFin erythrocyte-specific transcriptional activation. First, in vivo and in vitro analyses have been used to demonstrate that the level of activation by EKLF is dependent on the orientation and number of CACCC elements, that EKLF contains separable activation and DNA-binding domains, and that the EKLF proline rich region is a potent activator in CV-1 cells when fused to a nonrelated DNA-binding module. Second, we haveestablished a transient assay in murine erythroleukemia cells in which reproducible levels of a reporter can be induced when linked to a locus control region enhancer-beta-globin promoter and in which induction is abolished when the promoter CAC site is mutated to a GAL site. Third, we demonstrate that the EKLF transactivation region, when fused to the GAL DNA-binding domain, can restore inducibility to this mutatedconstruct and that this inducibility exhibits activator-, promoter-, and cell-type specificity. These results demonstrate that EKLF provides a crucial transactivation function for globin expression and furtherreinforce the idea that EKLF is an important regulator of CACCC element-directed transcription in erythroid cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 20:34:00