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Titolo:
ANALYSIS OF MAMMALIAN 20S PROTEASOME BIOGENESIS - THE MATURATION OF BETA-SUBUNITS IS AN ORDERED 2-STEP MECHANISM INVOLVING AUTOCATALYSIS
Autore:
SCHMIDTKE G; KRAFT R; KOSTKA S; HENKLEIN P; FROMMEL C; LOWE J; HUBER R; KLOETZEL PM; SCHMIDT M;
Indirizzi:
HUMBOLDT UNIV BERLIN,CHARITE,CTR MED EXPT,INST BIOCHEM,MONBIJOUSTR 2AD-10117 BERLIN GERMANY HUMBOLDT UNIV BERLIN,CHARITE,CTR MED EXPT,INST BIOCHEM D-10117 BERLINGERMANY MAX DELBRUCK CTR MOL MED D-13122 BERLIN GERMANY MAX PLANCK INST BIOCHEM,ABT STRUKTURFORSCH D-82152 MARTINSRIED GERMANY
Titolo Testata:
EMBO journal
fascicolo: 24, volume: 15, anno: 1996,
pagine: 6887 - 6898
SICI:
0261-4189(1996)15:24<6887:AOM2PB>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAJOR-HISTOCOMPATIBILITY-COMPLEX; MULTICATALYTIC PROTEINASE COMPLEX; PEPTIDE-HYDROLYZING ACTIVITY; THERMOPLASMA-ACIDOPHILUM; INTERFERON-GAMMA; YEAST PROTEASOME; LMP2; PROTEOLYSIS; UBIQUITIN; SEQUENCE;
Keywords:
AUTOCATALYTIC ACTIVATION; PROCESSING MECHANISM; PROTEASOME MATURATION; STRUCTURE FORMATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
62
Recensione:
Indirizzi per estratti:
Citazione:
G. Schmidtke et al., "ANALYSIS OF MAMMALIAN 20S PROTEASOME BIOGENESIS - THE MATURATION OF BETA-SUBUNITS IS AN ORDERED 2-STEP MECHANISM INVOLVING AUTOCATALYSIS", EMBO journal, 15(24), 1996, pp. 6887-6898

Abstract

Maturation of eukaryotic 20S proteasomes involves the processing of beta-subunits by limited proteolysis. To study the processing mechanismwe analysed different point mutations of the beta-subunit LMP2 in transfected human T2 cells, Here we show that the presence of the intact Gly-1Thr1 consensus motif and Lys33 are essential for correct processing, Mutation of Thr1, the active site residue in mature subunits, or of Lys33, results in complete inhibition of processing at the consensussite, In addition, proprotein processing irt vitro of wild-type LMP2,incorporated in immature 16S precursor complexes, can be blocked by aproteasome-specific inhibitor, While the processing of inhibitor-treated wild-type proprotein was completely prevented, the site-directed mutagenesis of LMP2 results in processing intermediates carrying an extension of 8-10 residues preceding Thr1, suggesting an additional cleavage event within the prosequence, Furthermore, exchange of mammalian prosequences interferes with processing efficiency and suggests subunitspecificity, Based on our data we propose a model for self-activationof proteasomal beta-subunits in which residue Thr1 serves as nucleophile and Lys33 as proton donor/acceptor, We provide evidence that subunit processing of mammalian beta-subunits proceeds via a novel ordered two-step mechanism involving autocatalysis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 10:38:25