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Titolo:
DETERMINATION OF SEMICARBAZIDE-SENSITIVE AMINE OXIDASE ACTIVITY IN HUMAN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUOROMETRICDETECTION
Autore:
VANDIJK J; BOOMSMA F; ALBERTS G; TVELD AJMI; SCHALEKAMP MADH;
Indirizzi:
ERASMUS UNIV ROTTERDAM,HOSP DIJKZIGT,INST CARDIOVASC RES,COEUR,DIV INTERNAL MED 1 3015 GD ROTTERDAM NETHERLANDS ERASMUS UNIV ROTTERDAM,HOSP DIJKZIGT,INST CARDIOVASC RES,COEUR,DIV INTERNAL MED 1 3015 GD ROTTERDAM NETHERLANDS
Titolo Testata:
Journal of chromatography B. Biomedical applications
fascicolo: 1, volume: 663, anno: 1995,
pagine: 43 - 50
Fonte:
ISI
Lingua:
ENG
Soggetto:
MONOAMINE-OXIDASE; RAT AORTA; UMBILICAL ARTERY; TRACE ANALYSIS; DEAMINATION; METHYLAMINE; ENZYME; METABOLISM; ALDEHYDES; TISSUES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
J. Vandijk et al., "DETERMINATION OF SEMICARBAZIDE-SENSITIVE AMINE OXIDASE ACTIVITY IN HUMAN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUOROMETRICDETECTION", Journal of chromatography B. Biomedical applications, 663(1), 1995, pp. 43-50

Abstract

We report here a simple and sensitive method for the measurement of semicarbazide-sensitive amine oxidase (SSAO) activity in human plasma. Benzaldehyde, generated during a 1-h incubation of plasma with benzylamine, is derivatized with the specific aldehyde reagent dimedone afterprior deproteinization. Quantitation of the derivatization product isdone by automated injection onto an isocratic high-performance liquidchromatographic system with fluorimetric detection. The assay shows good linearity and reproducibility (intra-assay C.V. 7%). Detection limit is 25 mU/l (= pmol/ml/min). In 51 healthy controls (age 49 +/- 13 yr, 20 males) the measured SSAO activity was 352 +/- 102 mU/l (mean +/-S.D.). A large number of samples (70-80) can easily be processed in one day by one technician.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 15:13:13