Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
USE OF RUBELLA-VIRUS E1 FUSION PROTEINS FOR DETECTION OF RUBELLA-VIRUS ANTIBODIES
Autore:
STARKEY WG; NEWCOMBE J; CORBETT KM; LIU KM; SANDERS PG; BEST JM;
Indirizzi:
UNITED MED & DENT SCH,DEPT VIROL,LAMBETH PALACE RD LONDON SE1 7EH ENGLAND UNIV SURREY,DEPT MICROBIOL & MOLEC SCI GUILDFORD GU2 5XH SURREY ENGLAND UNITED MED & DENT SCH,ST THOMAS HOSP,DEPT VIROL LONDON SE1 7EH ENGLAND
Titolo Testata:
Journal of clinical microbiology
fascicolo: 2, volume: 33, anno: 1995,
pagine: 270 - 274
SICI:
0095-1137(1995)33:2<270:UOREFP>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
LINKED-IMMUNOSORBENT-ASSAY; GLUTATHIONE-S-TRANSFERASE; LATEX AGGLUTINATION-TEST; ESCHERICHIA-COLI; MONOCLONAL-ANTIBODIES; STRUCTURAL PROTEINS; CAPSID PROTEIN; HEMAGGLUTINATION; NEUTRALIZATION; LOCALIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
W.G. Starkey et al., "USE OF RUBELLA-VIRUS E1 FUSION PROTEINS FOR DETECTION OF RUBELLA-VIRUS ANTIBODIES", Journal of clinical microbiology, 33(2), 1995, pp. 270-274

Abstract

Two glutathione S-transferase fusion proteins containing 44 (p1503) and 75 (p1509) amino acid residues of the rubella virus E1 glycoproteinwere expressed in Escherichia coli with the aim of producing a recombinant rubella virus antigen for use in serological assays, p1503 contained three neutralizing and hemagglutinating epitopes (G, M, Terry, L,M, Ho-Terry, P, Londesborough, and K, R, Rees, Arch, Virol, 98:189-197, 1988); p1509 contained the putative neutralization domain describedby Mitchell et al, (L, A, Mitchell, T, Zhang, M, Ho, D, Decarie, A, Tingle, M, Zrein, and M, Lacroix, J, Clin. Microbiol, 30:1841-1847, 1992) in addition to the three epitopes present in p1503, Both fusion proteins were soluble and affinity purified on glutathione-Sepharose 4B, In Western blots (immunoblots), p1503 and p1509 reacted with human sera containing rubella virus-specific immunoglobulin G, When used as antigens in indirect enzyme immunoassays to detect rubella virus-specificimmunoglobulin G, p1503 correctly identified the rubella virus antibody status of 43 (76.8%) and p1509 correctly identified that of 48 (85.7%) of 56 serum samples received for routine rubella virus antibody screening, The results obtained with p1509 compare well with those obtained with a latex agglutination assay,

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 05:33:02