Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
NEISSERIA PILC PROTEIN IDENTIFIED AS TYPE-4 PILUS TIP LOCATED ADHESIN
Autore:
RUDEL T; SCHEUERPFLUG I; MEYER TF;
Indirizzi:
MAX PLANCK INST BIOL,INFEKT BIOL ABT,SPEMANNSTR 34 D-72076 TUBINGEN GERMANY MAX PLANCK INST INFEKT BIOL D-10117 BERLIN GERMANY
Titolo Testata:
Nature
fascicolo: 6512, volume: 373, anno: 1995,
pagine: 357 - 359
SICI:
0028-0836(1995)373:6512<357:NPPIAT>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN EPITHELIAL-CELLS; ANTIGENIC VARIATION; ESCHERICHIA-COLI; VIBRIO-CHOLERAE; PHASE VARIATION; GONORRHOEAE; SEQUENCE; GENE; MENINGITIDIS; ORGANIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
27
Recensione:
Indirizzi per estratti:
Citazione:
T. Rudel et al., "NEISSERIA PILC PROTEIN IDENTIFIED AS TYPE-4 PILUS TIP LOCATED ADHESIN", Nature, 373(6512), 1995, pp. 357-359

Abstract

TYPE-4 pilus-mediated adherence of Neisseria gonorrhoeae and Neisseria meningitidis is considered to be a crucial early event in neisserialinfections(1,2). In addition to the principal pilus subunit (pilin orPilE), both pathogens produce low quantities of a phase-variable PilCprotein which is implicated in pilus biogenesis and pilus-mediated epithelial cell adherence(3,4). The identity, however, of the pilus adhesin has remained obscure(4,5). Here we describe the isolation of a PilC protein from a gonococcal overproducing strain and demonstrate its specific interaction with human epithelial cells. Our results are consistent with the cell and species tropisms of neisserial infections. Binding of PilC effectively competes with pilus-mediated, but not Opa-mediated(6), attachment of N. gonorrhoeae and of N. meningitidis, indicating that both pathogens interact with identical or very similar epithelial cell receptors. Immunogold electron microscopy using antisera raised against purified PilC and synthetic peptides locates PilC at the tip of gonococcal pill. PilC thus represents an essential pilus-associated adhesin, providing a rationale for selective protection against neisserial infections.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 11:33:18