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Titolo:
PROTEOLYTIC RELEASE OF HUMAN ANGIOTENSIN-CONVERTING ENZYME EXPRESSED IN CHINESE-HAMSTER OVARY CELLS IS ENHANCED BY PHORBOL ESTER
Autore:
EHLERS MRW; SCHOLLE RR; RIORDAN JF;
Indirizzi:
UNIV CAPE TOWN,SCH MED,DEPT BIOCHEM MED CAPE TOWN 7925 SOUTH AFRICA HARVARD UNIV,SCH MED,CTR BIOCHEM & BIOPHYS SCI & MED BOSTON MA 02115
Titolo Testata:
Biochemical and biophysical research communications
fascicolo: 2, volume: 206, anno: 1995,
pagine: 541 - 547
SICI:
0006-291X(1995)206:2<541:PROHAE>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMYLOID PRECURSOR PROTEIN; SECRETION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
18
Recensione:
Indirizzi per estratti:
Citazione:
M.R.W. Ehlers et al., "PROTEOLYTIC RELEASE OF HUMAN ANGIOTENSIN-CONVERTING ENZYME EXPRESSED IN CHINESE-HAMSTER OVARY CELLS IS ENHANCED BY PHORBOL ESTER", Biochemical and biophysical research communications, 206(2), 1995, pp. 541-547

Abstract

Membrane-bound angiotensin-converting enzyme (ACE) expressed in Chinese hamster ovary (CHO) cells is proteolytically released in soluble form into the medium. We find that this release is stimulated up to 50-fold by phorbol-12, 13-dibutyrate and also by the addition of fresh, serum-containing media. Concomitant with the enhanced release is a marked decrease in levels of membrane-bound ACE, down to 7% of resting revels in the case of phorbol eater stimulation. Staurosporine, a protein kinase C (PKC) inhibitor, abolishes the phorbol ester effect. Kinetic analysis of the stimulated release rate indicates that it is first order, likely due to substrate depletion; calculated half times, t1/2 are174 +/- 12 min and 40 +/- 6 min for the media-change and phorbol eater stimulated rates, respectively. Thus, release of membrane-bound ACE in CHO cells is regulated, in part, by a PKC-dependent mechanism. (C) 1995 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 06:36:25