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Titolo:
CLEAVAGE OF RECOMBINANT AND CELL-DERIVED HUMAN-IMMUNODEFICIENCY-VIRUS-1 (HIV-1) NEF PROTEIN BY HIV-1 PROTEASE
Autore:
GAEDIGKNITSCHKO K; SCHON A; WACHINGER G; ERFLE V; KOHLEISEN B;
Indirizzi:
GSF FORSCHUNGSZENTRUM UNWELT & GESUNDHEIT,INST MOLEK VIROL,INGOLSTAEDTER LANDSTR 1 D-85764 OBERSCHLEISSHEIM GERMANY GSF FORSCHUNGSZENTRUM UNWELT & GESUNDHEIT,INST MOLEK VIROL D-85764 OBERSCHLEISSHEIM GERMANY
Titolo Testata:
FEBS letters
fascicolo: 3, volume: 357, anno: 1995,
pagine: 275 - 278
SICI:
0014-5793(1995)357:3<275:CORACH>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
TYPE-1 PROTEASE; MUTATIONAL ANALYSIS; LOCALIZATION; REPLICATION; ASTROCYTES; ANTIBODY; PEPTIDE; REGION; LTR;
Keywords:
NEF PROTEIN; HIV-1 PROTEASE; PROTEOLYTIC CLEAVAGE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
27
Recensione:
Indirizzi per estratti:
Citazione:
K. Gaedigknitschko et al., "CLEAVAGE OF RECOMBINANT AND CELL-DERIVED HUMAN-IMMUNODEFICIENCY-VIRUS-1 (HIV-1) NEF PROTEIN BY HIV-1 PROTEASE", FEBS letters, 357(3), 1995, pp. 275-278

Abstract

Recombinant purified Nef protein of HIV-1, as well as Nef protein derived from extracts of permanently HIV-1 infected glioblastoma cells and monocytes, are specifically cleaved by the HIV-1 protease. Nef cleavage products in cellular extracts treated with protease showed identical molecular weights as those obtained by digestion of purified Nef with recombinant HIV-1 protease. Since cellular extracts were prepared by detergent and mechanical lysis it cannot be excluded that physiological cytoplasmic conditions were altered. The lack of Nef cleavage by endogenous HIV-1 protease in infected cells might be due to low concentrations of viral protease and the presence of Gag precursor molecules as natural substrate. Using a panel of monoclonal antibodies two cleavage fragments of 19 kDa and 8 kDa were defined. The cleavage site was located by microsequencing between amino acid 57 and 58 (AW()LEAQEEEEVGF). The conserved cleavage motif within HIV-1 Nef suggests a potential biological function of Nef processing.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 02:17:41