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Titolo:
SITE-SPECIFIC INTEGRATION OF THE PHAGE PHI-CTX GENOME INTO THE PSEUDOMONAS-AERUGINOSA CHROMOSOME - CHARACTERIZATION OF THE FUNCTIONAL INTEGRASE GENE LOCATED CLOSE TO AND UPSTREAM OF ATTP
Autore:
WANG Z; XIONG G; LUTZ F;
Indirizzi:
UNIV GIESSEN KLINIKUM,INST PHARMAKOL & TOXIKOL,FRANKFURTER STR 107 D-35392 GIESSEN GERMANY
Titolo Testata:
MGG. Molecular & general genetics
fascicolo: 1, volume: 246, anno: 1995,
pagine: 72 - 79
SICI:
0026-8925(1995)246:1<72:SIOTPP>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYTOTOXIN; EXPRESSION;
Keywords:
PSEUDOMONAS AERUGINOSA; BACTERIOPHAGE-PHI-CTX; SITE-SPECIFIC INTEGRATION; INTEGRASE GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
11
Recensione:
Indirizzi per estratti:
Citazione:
Z. Wang et al., "SITE-SPECIFIC INTEGRATION OF THE PHAGE PHI-CTX GENOME INTO THE PSEUDOMONAS-AERUGINOSA CHROMOSOME - CHARACTERIZATION OF THE FUNCTIONAL INTEGRASE GENE LOCATED CLOSE TO AND UPSTREAM OF ATTP", MGG. Molecular & general genetics, 246(1), 1995, pp. 72-79

Abstract

The site-specific integration of the phage phi CTX genome, which carries the gene for a pore-forming cytotoxin, into the Pseuodomonas aeruginosa chromosome was analysed. The 1,167 bp integrase gene, int, located immediately upstream of the attachment site, attP, was characterized using plasmid constructs, harbouring the integration functions, and serving as an integration probe in both P. aeruginosa and Escherichia coli. The attP plasmids p1000/p400 in the presence of the int plasmid pIBH and attP-int plasmids pINT/pINTS can be stably integrated into the P. aeruginosa chromosome. Successful recombination between the attP plasmid p1000 and the attB plasmid p5.1, in the presence of the int plasmid pIBH in E. coli HB101 showed that the int gene is active in trans in E. coli. The int gene product was detected as a 43 kDa protein inE. coli maxicells harbouring pINT. Proposed integration arm regions downstream of attP are not necessary for the integration process. pINT and phage phi CTX could be integrated together into P. aeruginosa chromosomal DNA, yielding double integrates.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 00:50:20