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Titolo:
ALCOHOL-DEHYDROGENASE FROM HUMAN STOMACH - VARIABILITY IN NORMAL MUCOSA AND EFFECT OF AGE, GENDER, ADH(3), PHENOTYPE AND GASTRIC REGION
Autore:
MORENO A; PARES A; ORTIZ J; ENRIQUEZ J; PARES X;
Indirizzi:
UNIV AUTONOMA BARCELONA,FAC SCI,DEPT BIOCHEM & MOLEC BIOL E-08193 BARCELONA SPAIN UNIV AUTONOMA BARCELONA,FAC SCI,DEPT BIOCHEM & MOLEC BIOL E-08193 BARCELONA SPAIN HOSP CLIN BARCELONA,ALCOHOL UNIT E-08036 BARCELONA SPAIN HOSP CLIN BARCELONA,LIVER UNIT E-08036 BARCELONA SPAIN HOSP SANTA CRUZ & SAN PABLO,SERV GASTROENTEROL E-08025 BARCELONA SPAIN
Titolo Testata:
Alcohol and alcoholism
fascicolo: 6, volume: 29, anno: 1994,
pagine: 663 -
SICI:
0735-0414(1994)29:6<663:AFHS-V>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
ALDEHYDE DEHYDROGENASES; 1ST-PASS METABOLISM; ETHANOL; PURIFICATION; ENZYME;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
22
Recensione:
Indirizzi per estratti:
Citazione:
A. Moreno et al., "ALCOHOL-DEHYDROGENASE FROM HUMAN STOMACH - VARIABILITY IN NORMAL MUCOSA AND EFFECT OF AGE, GENDER, ADH(3), PHENOTYPE AND GASTRIC REGION", Alcohol and alcoholism, 29(6), 1994, pp. 663

Abstract

Alcohol dehydrogenase (ADH) has been analysed in 36 endoscopic biopsies of normal gastric body and/or antrum mucosa, from 31 individuals with an age between 17 and 79 years. Oesophageal, duodenal and oral mucosa specimens have been also examined. Stomach mucosa contains three isozyme types: the gamma gamma-ADH forms (class I), sigma sigma-ADH (class IV) and chi chi-ADH (class III). gamma gamma-ADH was present in allgastric samples, while sigma sigma-ADH was detected in all body specimens (n = 15) but only in eight of 20 antrum biopsies. The presence ofhigh sigma sigma-ADH activity in oral and oesophageal mucosa confirmsthe distribution of class IV in the upper gastrointestinal tract where it may serve as a first metabolic barrier against ingested alcohols and aldehydes. Considering all gastric specimens, ADH activity was 5.78 +/- 2.61 mU/mg of protein with 100 mM ethanol, pH 10.0. ADH activitywas higher in men than women and in gastric body than in antrum, although differences did not reach statistical significance. However, activity was significantly higher in subjects below 50 years than those older than 50 years. Furthermore, an inverse correlation was found between gastric ADH activity and age (r = -0.40, P < 0.02). In old subjectsADH activity was significantly higher in gastric body (5.87 +/- 1.22 mU/mg) than in antrum (4.14 +/- 1.78 mU/mg) (P = 0.03). Differences inactivity between samples from each ADH(3) phenotype were statistically not significant but corresponded to those expected from the kinetic constants of the respective gamma gamma-ADH isozymes, which suggests that ADH(3) polymorphism affects activity. We conclude that age has a major effect on gastric ADH, while stomach region and ADH(3) phenotype also influence its activity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 14:49:08