Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
IMMUNOGENICITY OF THE CHIMERIC PLASMINOGEN-ACTIVATOR K2TU-PA IN RABBITS
Autore:
ASSELBERGS FAM;
Indirizzi:
CIBA GEIGY LTD,DEPT BIOTECHNOL,K681442 CH-4002 BASEL SWITZERLAND
Titolo Testata:
Blood coagulation & fibrinolysis
fascicolo: 6, volume: 5, anno: 1994,
pagine: 913 - 919
SICI:
0957-5235(1994)5:6<913:IOTCPK>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
N-GLYCOLYLNEURAMINIC ACID; HAMSTER OVARY CELLS; TISSUE-TYPE; THROMBOLYTIC ACTIVITY; CARBOHYDRATE CHAINS; HEPATIC RECEPTOR; PRO-UROKINASE; RECOMBINANT; STREPTOKINASE; ANTIBODIES;
Keywords:
PLASMINOGEN ACTIVATORS; TISSUE-TYPE PLASMINOGEN ACTIVATOR; UROKINASE; THROMBOLYTIC AGENTS; THROMBOSIS; KRINGLE STRUCTURES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
F.A.M. Asselbergs, "IMMUNOGENICITY OF THE CHIMERIC PLASMINOGEN-ACTIVATOR K2TU-PA IN RABBITS", Blood coagulation & fibrinolysis, 5(6), 1994, pp. 913-919

Abstract

Rabbits were repeatedly immunized with a chimeric plasminogen activator composed of the kringle-2 domain of human tissue-type plasminogen activator (t-PA) attached to the serine protease domain of the human urokinase-type plasminogen activator (u-PA). IgG from these rabbits was purified, biotinylated and subjected to affinity chromatography on K2tu-PA covalently attached to Sepharose. Roughly half the antibodies recovered from the K2tu-PA column could subsequently be bound to a columnwith similarly immobilized t-PA, whereas the other half bound to a u-PA column. Less than 0.01% of the biotinylated anti-K2tu-PA antibodiesdid not bind to either t-PA or u-PA and perhaps are directed against neoantigenic determinants on K2tu-PA, not present in the natural plasminogen activators.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 00:51:20