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Titolo:
BACTERIAL MUTAGENICITY OF CIGARETTE-SMOKE AND ITS INTERACTION WITH ETHANOL
Autore:
DEFLORA S; BALANSKY R; GASPARINI L; CAMOIRANO A;
Indirizzi:
UNIV GENOA,INST HYG & PREVENT MED,VIA PASTORE 1 I-16132 GENOA ITALY NATL ONCOL CTR BU-1756 SOFIA BULGARIA
Titolo Testata:
Mutagenesis
fascicolo: 1, volume: 10, anno: 1995,
pagine: 47 - 52
SICI:
0267-8357(1995)10:1<47:BMOCAI>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
TOBACCO-SMOKE; SALMONELLA; NITROARENES; CONDENSATE; GENOTOXICITY; CHEMICALS; STRAINS; URINE; DNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
S. Deflora et al., "BACTERIAL MUTAGENICITY OF CIGARETTE-SMOKE AND ITS INTERACTION WITH ETHANOL", Mutagenesis, 10(1), 1995, pp. 47-52

Abstract

The mutagenicities of mainstream cigarette smoke (CS), a cigarette smoke condensate (CSC) and smokers' urines were investigated by using batteries of Salmonella typhimurium and Escherichia coil strains. The S9-mediated mutagenicity of CSC was remarkably enhanced when using nitroreductase- and especially O-acetyltransferase-overproducing derivatives of the classical strains TA98 and TA100, with the following rank of sensitivity: YG1024 > YG1029 > YG1021 > TA98NR > YG1026 > TA98 > TA100> TA100-DNP6 > TA100NR > TA98-1,8-DNP6. With YG1024, a doubling of spontaneous revertants was observed with as little as 1/110 of the smokecondensate recovered from one cigarette under our experimental conditions. Similarly, the SE-mediated mutagenicity of mainstream CS was considerably increased in YG1024 and YG1029, the O-acetyltransferase-overproducing derivatives of TA98 and TA100, respectively. In the absence of S9 mix, the concentrates of 23 urine specimens from five smokers failed to revert S. typhimurium TA98 and YG1024, and were equitoxic in E. coli WP2 and its repair-deficient counterpart CM871 (uvrA(-), recA(-), lexA(-)). In the presence of S9 mix, all specimens were mutagenic, with an average YG1024:TA98 ratio of 6.6:1. These patterns suggest that the bacterial mutagenicity of smoke-associated complex mixtures is mainly due to aromatic amines. The mutagenicities of other typical constituents of CS, i,e, the polycyclic aromatic hydrocarbon benzo[a]pyrene (BP) and the tobacco specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), were not appreciably enhanced in the O-acetyltransferase-overproducing strain YG1029, compared to its parental strain TA100. Moreover, BP and NNK induced less than additive mutagenicresponses when combined at high doses. It was possible to reproduce in vitro the synergism between CS and ethanol by exposing agar plates, incorporating bacteria (YG1024 or YG1029), S9 mix and ethanol, to mainstream CS. With YG1024, a considerable enhancement of CS mutagenicity was observed with as low as 5 mu I volumes of ethanol. No synergism could be conversely detected by using the classical tester strains TA98 and TA100.

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Documento generato il 21/09/20 alle ore 02:35:28