Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
IN-VITRO FLUID SECRETION BY EPITHELIUM FROM POLYCYSTIC KIDNEYS
Autore:
GRANTHAM JJ; YE M; GATTONE VH; SULLIVAN LP;
Indirizzi:
UNIV KANSAS,MED CTR,DEPT MED,DIV NEPHROL & HYPERTENS,SUDLER 4015,3901RAINBOW BLVD KANSAS CITY KS 66160 UNIV KANSAS,MED CTR,DEPT ANAT & CELL BIOL KANSAS CITY KS 66160 UNIV KANSAS,MED CTR,DEPT PHYSIOL KANSAS CITY KS 66160 UNIV KANSAS,MED CTR,KIDNEY & UROL RES CTR KANSAS CITY KS 66160
Titolo Testata:
The Journal of clinical investigation
fascicolo: 1, volume: 95, anno: 1995,
pagine: 195 - 202
SICI:
0021-9738(1995)95:1<195:IFSBEF>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYST FORMATION; RENAL CYSTS; K+-ATPASE; DISEASE; PROLIFERATION; MECHANISMS; TRANSPORT; INVITRO; GROWTH; CELLS;
Keywords:
CYST; SECRETION; CATION TRANSPORT; ANION TRANSPORT; FLUID TRANSPORT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
J.J. Grantham et al., "IN-VITRO FLUID SECRETION BY EPITHELIUM FROM POLYCYSTIC KIDNEYS", The Journal of clinical investigation, 95(1), 1995, pp. 195-202

Abstract

The size of the kidneys in patients with autosomal dominant polycystic kidney disease (ADPKD) is due in large measure to the accumulation of secreted fluid within thin-walled epithelial sacs. We measured the net transepithelial movement of liquid in response to forskolin in isolated, intact cysts excised from the surface of human ADPKD kidneys andin cultured, polarized monolayers of epithelial cells derived from ADPKD cysts, 10 excised cysts bathed symmetrically in control culture medium secreted fluid at a rate of 0.19+/-0.03 mu l/cm(2) per hour afterstimulation with forskolin (10 mu M). Ouabain (100 mu M) addition to the cavity fluid did not change the rate of fluid secretion of 10 forskolin-treated cysts, but addition of the glycoside to the external bathing medium fluid of nine cysts decreased secretion to -0.004+/-0.05 mu l/cm(2) per hour, 24 monolayers absorbed fluid (range -0.029 to -0.412 mu l/cm(2) per hour); by contrast, fluid was secreted (range 0.074 to 1.242 mu l/cm(2) per hour) after stimulation with forskolin (10 mu M). Ouabain (0.1 mu M) in the basolateral but not in the apical mediuminhibited fluid secretion. Forskolin increased the intracellular cyclic AMP content of ADPKD and MDCK monolayers by 236 and 196%, respectively. Six ADPKD monolayers had stable lumen negative transepithelial electrical potential differences (PDte) of -1.4+/-0.3 mV, positive shortcircuit currents (SCC) of 11.9+/-2.1 mu Amp/cm(2) and a tissue resistance (R(te)) of 116+/-14 ohm.cm(2). Forskolin increased SCC to 15.5+/-1.9 mu Amp/cm(2) (P < 0.005) and decreased R(te) to 95+/-13 ohm.cm(2) (P < 0.05); PDte remained stable at -1.4+/-0.3 mV. Ouabain (10 mu M) had no effect when added to the apical medium, but in the basolateral medium decreased SCC to 1.7+/-0.3 mu Amp/cm(2) and PDte to -0.2+/-0.1 mV. We conclude that ADPKD cells in surface cysts have the potential toabsorb or to secrete solutes and fluid, cAMP-mediated fluid secretionfrom the basolateral medium into the lumen of surface ADPKD cysts maybe driven by anion transport.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 04:29:06