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Titolo:
MICROSCALE IMMOBILIZED PROTEASE REACTOR COLUMNS FOR PEPTIDE-MAPPING BY LIQUID-CHROMATOGRAPHY MASS-SPECTRAL ANALYSES
Autore:
DAVIS MT; LEE TD; RONK M; HEFTA SA;
Indirizzi:
CITY HOPE NATL MED CTR,BECKMAN RES INT,DIV IMMUNOL DUARTE CA 91010
Titolo Testata:
Analytical biochemistry
fascicolo: 1, volume: 224, anno: 1995,
pagine: 235 - 244
SICI:
0003-2697(1995)224:1<235:MIPRCF>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
WATER-SOLUBLE CARBODIIMIDE; AMINO-ACID-SEQUENCE; MASS-SPECTROMETRY; DIGESTION; PROTEINS; ELECTROPHORESIS; OPTIMIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
18
Recensione:
Indirizzi per estratti:
Citazione:
M.T. Davis et al., "MICROSCALE IMMOBILIZED PROTEASE REACTOR COLUMNS FOR PEPTIDE-MAPPING BY LIQUID-CHROMATOGRAPHY MASS-SPECTRAL ANALYSES", Analytical biochemistry, 224(1), 1995, pp. 235-244

Abstract

Methods and procedures for the construction and operation of a microscale immobilized protease (trypsin) reactor are described. Optimization of reactor efficiency with regard to reactor flowrate, length, and temperature as well as sample concentration has been examined, and a novel system for sample preconcentration is presented. The structural analysis of a standard protein (cytochrome c) by a combination of trypsin microreactor digestion followed by off-line electrospray ionization MS analysis is demonstrated at the 10-pmol level. The application of these techniques for the rapid confirmation (1-2 h) of a genetic variant (hemoglobin E) including MS/MS analysis of the variant peptide is included. (C) 1995 Academic Press, Inc.

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Documento generato il 24/09/20 alle ore 06:16:29