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Titolo:
B-50 GROWTH-ASSOCIATED PROTEIN-43, A MARKER OF NEURAL DEVELOPMENT IN XENOPUS-LAEVIS/
Autore:
SCHRAMA LH; LEPPERDINGER G; MORITZ A; VANDENENGEL NK; MARQUART A; OESTREICHER AB; EGGEN BJL; HAGE WJ; RICHTER K; DESTREE OHJ;
Indirizzi:
UNIV UTRECHT,RUDOLF MAGNUS INST NEUROSCI,PHYSIOL CHEM LAB,UNIV WEG 100 NL-3584 CG UTRECHT NETHERLANDS UNIV UTRECHT,RUDOLF MAGNUS INST NEUROSCI NL-3584 CG UTRECHT NETHERLANDS SALZBURG UNIV,INST GENET & ALLGEMEINE BIOL A-5020 SALZBURG AUSTRIA NETHERLANDS INST DEV BIOL,HUBRECHT LAB NL-3584 CT UTRECHT NETHERLANDS
Titolo Testata:
Neuroscience
fascicolo: 2, volume: 76, anno: 1997,
pagine: 635 - 652
SICI:
0306-4522(1997)76:2<635:BGPAMO>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-ASSOCIATED PROTEIN; GAP-43 GENE-EXPRESSION; AMINO-ACID-SEQUENCE; KINASE-C SUBSTRATE; CALMODULIN-BINDING PROTEIN; DEVELOPING NERVOUS-SYSTEM; B-50 GAP-43; RAT-BRAIN; PHOSPHORYLATION SITE; MESSENGER-RNA;
Keywords:
B-50/GAP-43; XENOPUS LAEVIS; DEVELOPMENT; CDNA; IMMUNOCYTOCHEMISTRY; IN SITU HYBRIDIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
81
Recensione:
Indirizzi per estratti:
Citazione:
L.H. Schrama et al., "B-50 GROWTH-ASSOCIATED PROTEIN-43, A MARKER OF NEURAL DEVELOPMENT IN XENOPUS-LAEVIS/", Neuroscience, 76(2), 1997, pp. 635-652

Abstract

To study the regulation and function of the growth-associated proteinB-50/growth-associated protein-43 (mel. wt 43,000) in Xenopus laevis,B-50/growth-associated protein-43 complementary DNAs were isolated and characterized. The deduced amino acid sequence revealed potential functional domains of Xenopus B-50/growth-associated protein-43 that maybe involved in G-protein interaction, membrane binding, calmodulin-binding and protein kinase C phosphorylation. The expression of B-50/growth-associated protein-43 at the RNA and protein level during development was investigated using the Xenopus complementary DNA and the monoclonal B-50/growth-associated protein-43 antibody NM2. The antibody NM2recognized the gene product on western blot and in whole-mount immunocytochemistry of Xenopus embryos. Moreover, visualization of the developmentally regulated appearance of B-50/growth-associated protein-43 immunoreactivity showed that this mode of detection may be used to monitor axonogenesis under various experimental conditions. In the adult Xenopus, XB-50/growth-associated protein-43 messenger RNA was shown to be expressed at high levels in brain, spinal cord and eye using northern blotting. The earliest expression detected on northern blot was at developmental stage 13 with poly(A) RNA. By whole-mount immunofluorescence, applying the confocal laser scanning microscope, the protein wasfirst detected in embryos From stage 20, where it was expressed in the developing trigeminal ganglion. Also later in development the expression of the B-50/growth-associated protein-33 gene was restricted to the nervous system in Xenopus, as was previously Found for the mouse. In conclusion, we find that XB-50/growth-associated protein-33 is a good marker to study the development of the nervous system in Xenopus laevis. Copyright (C) 1996 IBRO.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 07:56:31