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Titolo:
DIFFERENTIATION-DEPENDENT EXPRESSION OF THE BCL-2 PROTOONCOGENE IN THE HUMAN TROPHOBLAST LINEAGE
Autore:
SAKURAGI N; MATSUO H; COUKOS G; FURTH EE; BRONNER MP; VANARSDALE CM; KRAJEWSKY S; REED JC; STRAUSS JF;
Indirizzi:
UNIV PENN,SCH MED,DEPT OBSTET & GYNECOL,778 CLIN RES BLDG,422 CURIE BLVD PHILADELPHIA PA 19104 UNIV PENN,SCH MED,DEPT OBSTET & GYNECOL PHILADELPHIA PA 19104 UNIV PENN,SCH MED,DEPT PATHOL & LAB MED PHILADELPHIA PA 19104 LA JOLLA CANC RES INST,LA JOLLA CANC RES FDN LA JOLLA CA 92161
Titolo Testata:
Journal of the Society for Gynecologic Investigation
fascicolo: 2, volume: 1, anno: 1994,
pagine: 164 - 172
SICI:
1071-5576(1994)1:2<164:DEOTBP>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROGRAMMED CELL-DEATH; MESSENGER-RNA; MEMBRANE PROTEIN; LYMPHOID-TISSUES; PROTOONCOGENE; PREVENTION; P53;
Keywords:
PROTOONCOGENE; BCL-2; APOPTOSIS; TROPHOBLAST; CHORIOCARCINOMA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
22
Recensione:
Indirizzi per estratti:
Citazione:
N. Sakuragi et al., "DIFFERENTIATION-DEPENDENT EXPRESSION OF THE BCL-2 PROTOONCOGENE IN THE HUMAN TROPHOBLAST LINEAGE", Journal of the Society for Gynecologic Investigation, 1(2), 1994, pp. 164-172

Abstract

OBJECTIVE: We explored the role of the BCL-2 proto-oncogene in the life cycle of trophoblast cells by examining: 1) the patterns of BCL-2 expression in normal placenta at various gestational ages and in specimens of hydatidiform moles and choriocarcinomas, and 2) the effects of cyclic adenosine monophosphate (cAMP) treatment of JEG-3 choriocarcinoma cells, which induces differentiated functions, on BCL-2. METHODS: BCL-2 protein was localized by indirect immunofluorescence and immunoperoxidase staining of tissue sections and cells using monoclonal and polyclonal antibodies. Western and Northern blotting were used to assessBCL-2 and p53 protein and mRNA levels, respectively. JEG-3 cells weretransfected with a BCL-2 expression plasmid to establish that BCL-2 protein could be expressed at high levels in this cell type. RESULTS: BCL-2 immunostaining was most prominent in the syncytiotrophoblast of normal placenta. It was found in syncytiotrophoblast of complete and partial hydatidiform moles, whereas cytotrophoblast staining was weak. BCL-2 immunostaining was also barely detectable in choriocarcinoma cells (JEG-3 cells) and a primary choriocarcinoma. However, BCL-2 protein could be transiently overexpressed in JEG-3 cells by transfection withan expression plasmid. Western blot analysis revealed low levels of BCL-2 in JEG-3 cells and a rise in BCL-2 protein in placental extracts from 10 weeks' gestation, but at low term. BCL-2 transcripts were substantially more abundant in term placenta than in JEG-3 cells. Treatment of JEG-3 cells with 8-Br-cAMP, which induces genes characteristic ofthe syncytiotrophoblast, raised BCL-2 protein approximately twofold, whereas p53 mRNA declined. CONCLUSIONS: We conclude that: 1) There is a differentiation-dependent pattern of BCL-2 expression in the placenta, with the protein being most abundant in terminally differentiated trophoblast cells; 2) there appears to be an inverse relation between BCL-2 and p53 expression in trophoblast; and 3) cAMP regulates BCL-2 protein in trophoblast cells. We speculate that the expression of BCL-2 in terminally differentiated trophoblast cells, and hence resistance to apoptotic cell death, may be one mechanism by which trophoblast massis preserved during pregnancy. Conversely, the relatively low expression of BCL-2 in choriocarcinoma cells may render them more susceptibleto apoptosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 18:08:34